Chitosan-collagen-cerium hydroxyapatite nanocomposites for In-vitro gentamicin drug delivery and antibacterial properties

IF 3.1 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY
{"title":"Chitosan-collagen-cerium hydroxyapatite nanocomposites for In-vitro gentamicin drug delivery and antibacterial properties","authors":"","doi":"10.1016/j.cartre.2024.100392","DOIUrl":null,"url":null,"abstract":"<div><p>The controlled release of antibiotics is crucial to improving antimicrobial efficacy, reducing the risk of bacterial resistance, and ensuring a localized therapeutic effect. In this work, <em>In-vitro</em> Gentamicin release was studied using fluorescence chitosan collagen-cerium hydroxyapatite nanocomposites. Cerium-hydroxyapatite nanoparticles were synthesized using the hydrothermal method, and the nanocomposites were prepared by mixing chitosan-collagen-cerium hydroxyapatite at different weight ratios. Structural characterization was conducted using scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, and fluorescence microscopy. Ultraviolet–visible spectroscopy (UV–Vis) was used to quantify the release of gentamicin in simulated body fluid. Results showed that hydroxyapatite releases 90 % of gentamicin in the first 10 min, and the Chitosan-collagen-cerium hydroxyapatite nanocomposites release 80 % of gentamicin after 2 h. The antibacterial activity was studied against <em>Escherichia coli (E. coli)</em> at different time intervals. These nanocomposites can potentially improve the performance of biomedical applications.</p></div>","PeriodicalId":52629,"journal":{"name":"Carbon Trends","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667056924000737/pdfft?md5=a9b58f9bd163a94a053fcecfd203a167&pid=1-s2.0-S2667056924000737-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbon Trends","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667056924000737","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

Abstract

The controlled release of antibiotics is crucial to improving antimicrobial efficacy, reducing the risk of bacterial resistance, and ensuring a localized therapeutic effect. In this work, In-vitro Gentamicin release was studied using fluorescence chitosan collagen-cerium hydroxyapatite nanocomposites. Cerium-hydroxyapatite nanoparticles were synthesized using the hydrothermal method, and the nanocomposites were prepared by mixing chitosan-collagen-cerium hydroxyapatite at different weight ratios. Structural characterization was conducted using scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, and fluorescence microscopy. Ultraviolet–visible spectroscopy (UV–Vis) was used to quantify the release of gentamicin in simulated body fluid. Results showed that hydroxyapatite releases 90 % of gentamicin in the first 10 min, and the Chitosan-collagen-cerium hydroxyapatite nanocomposites release 80 % of gentamicin after 2 h. The antibacterial activity was studied against Escherichia coli (E. coli) at different time intervals. These nanocomposites can potentially improve the performance of biomedical applications.

用于庆大霉素体外给药和抗菌的壳聚糖-胶原-羟基磷灰石铈纳米复合材料
抗生素的控制释放对于提高抗菌效果、降低细菌耐药性风险和确保局部治疗效果至关重要。本研究利用荧光壳聚糖胶原-羟磷灰石铈纳米复合材料对庆大霉素的体外释放进行了研究。铈-羟基磷灰石纳米粒子采用水热法合成,纳米复合材料由不同重量比的壳聚糖-胶原蛋白-羟基磷灰石铈混合制备而成。利用扫描电子显微镜、透射电子显微镜、傅立叶变换红外光谱、拉曼光谱和荧光显微镜进行了结构表征。紫外可见光谱(UV-Vis)用于量化庆大霉素在模拟体液中的释放量。结果表明,羟基磷灰石在最初的 10 分钟内释放了 90% 的庆大霉素,壳聚糖-胶原-铈羟基磷灰石纳米复合材料在 2 小时后释放了 80% 的庆大霉素。这些纳米复合材料有望改善生物医学应用的性能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Carbon Trends
Carbon Trends Materials Science-Materials Science (miscellaneous)
CiteScore
4.60
自引率
0.00%
发文量
88
审稿时长
77 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信