Combinations of bacterial probiotics and yeast postbiotics influence fat deposition and growth in the nematode C. elegans

Abstract

Background

Probiotics are live microorganisms with intended benefits on human health including obesity. As a small and fast-growing whole organism model, Caenorhabditis elegans has been used to assess the health effects of probiotics where mechanisms can be assessed through available genetic tools. Results from C. elegans can provide data on the effect of specific probiotic strains and combinations with prebiotics and postbiotics on health-related physiology to inform selections of interventions for further study. We hypothesized that specific combinations with prebiotics and postbiotics could both speed up worm development and reduce fat deposition, suggesting they allow for more effective nutrient utilization.

Methods

Here we expose C. elegans to the ABB S20 strain of Lactiplantibacillus plantarum in combination with different prebiotics and postbiotics. We then measure how these affect growth and development speed as well as fat deposition by measuring the time until the appearance of progeny and measuring Oil Red O staining respectively.

Results

Our results show that the combination of probiotic L. plantarum ABB S20 plus the postbiotic inactive yeasts K. marxianus ABB S8 and S. boulardii ABB S3 resulted in fast growth and reduced fat deposition compared to L. plantarum ABB S20 alone.

Conclusion

These results demonstrate the usefulness of C. elegans as a model to efficiently screen though combinations of probiotics, prebiotics and postbiotics to find those that are candidates to help with effective nutrition use and therefore weight management.