Expression of Kielin/chordin-like protein is regulated by BMP-2 in osteoblasts

IF 2.1 Q3 ENDOCRINOLOGY & METABOLISM

Bone Reports Pub Date : 2024-07-20 DOI:10.1016/j.bonr.2024.101793

Kazuki Toba , Atsushi Yamada , Kiyohito Sasa , Tatsuo Shirota , Ryutaro Kamijo

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引用次数: 0

Abstract

Bone morphogenetic protein (BMP), an osteoinductive factor, is a cytokine that induces osteoblast differentiation and mineralization, and expected to be applicable for hard tissue reconstruction. Kielin/chordin-like protein (Kcp), a member of the family of cysteine-rich proteins, enhances BMP signaling. The present study found that expression of Kcp in osteoblasts was induced by BMP-2 in a concentration- and time-dependent manner. Up-regulation of Kcp by BMP-2 was inhibited by Dorsomorphin, a SMAD signaling inhibitor. The involvement of up-regulation of Kcp by BMP-2 in induction of osteoblast differentiation by BMP-2 was also examined, which showed that suppression of Kcp expression by si Kcp partially inhibited induction of osteoblast differentiation and mineralization by BMP-2. Together, these results suggest that Kcp induced by BMP-2 functions to provide positive feedback for promotion of osteoblastic differentiation and mineralization by BMP-2 in osteoblasts.

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成骨细胞中 Kielin/chordin-like 蛋白的表达受 BMP-2 调节

骨形态发生蛋白（BMP）是一种骨诱导因子，是一种诱导成骨细胞分化和矿化的细胞因子，有望用于硬组织重建。Kielin/chordin-like蛋白（Kcp）是富半胱氨酸蛋白家族的一员，能增强BMP信号转导。本研究发现，成骨细胞中 Kcp 的表达是由 BMP-2 以浓度和时间依赖性方式诱导的。BMP-2对Kcp的上调作用被SMAD信号抑制剂多索吗啡（Dorsomorphin）所抑制。研究还考察了 BMP-2 诱导成骨细胞分化过程中 Kcp 上调的参与情况，结果表明 si Kcp 可部分抑制 BMP-2 诱导成骨细胞分化和矿化。这些结果表明，BMP-2诱导的Kcp为BMP-2促进成骨细胞分化和矿化提供了正反馈。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bone Reports Medicine-Orthopedics and Sports Medicine

CiteScore

4.30

自引率

4.00%

发文量

444

审稿时长

57 days

期刊介绍： Bone Reports is an interdisciplinary forum for the rapid publication of Original Research Articles and Case Reports across basic, translational and clinical aspects of bone and mineral metabolism. The journal publishes papers that are scientifically sound, with the peer review process focused principally on verifying sound methodologies, and correct data analysis and interpretation. We welcome studies either replicating or failing to replicate a previous study, and null findings. We fulfil a critical and current need to enhance research by publishing reproducibility studies and null findings.