K. V. Serebrennikova, L. V. Barshevskaya, A. V. Zherdev, B. B. Dzantiev
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Abstract
The aim of the study was to increase the sensitivity of immunochromatographic assay (ICA), achieved using gold nanorods modified with 5,5'-dithiobis-(2-nitrobenzoic acid). The resulting nanomarker makes it possible to quantify the target analyte using colorimetry and surface-enhanced Raman spectroscopy (SERS). One of the widespread mycotoxins, ochratoxin A (OTA), was chosen as the object of study. After functionalization of gold nanorods with antispecies antibodies, the SERS-active tag was used to detect immune complexes formed in the analytical zone as a result of the competitive interaction between free OTA and the hapten-protein conjugate for the binding sites of specific antibodies. After immunochromatographic assay, SERS intensity of 5,5'-dithiobis-(2-nitrobenzoic acid) was measured. Under optimized conditions, the detection limit of OTA in the colorimetric detection mode was 1.7 ng/mL, and when recording ICA results using SERS, it decreased to 33 pg/mL.
期刊介绍：
Nanobiotechnology Reports publishes interdisciplinary research articles on fundamental aspects of the structure and properties of nanoscale objects and nanomaterials, polymeric and bioorganic molecules, and supramolecular and biohybrid complexes, as well as articles that discuss technologies for their preparation and processing, and practical implementation of products, devices, and nature-like systems based on them. The journal publishes original articles and reviews that meet the highest scientific quality standards in the following areas of science and technology studies: self-organizing structures and nanoassemblies; nanostructures, including nanotubes; functional and structural nanomaterials; polymeric, bioorganic, and hybrid nanomaterials; devices and products based on nanomaterials and nanotechnology; nanobiology and genetics, and omics technologies; nanobiomedicine and nanopharmaceutics; nanoelectronics and neuromorphic computing systems; neurocognitive systems and technologies; nanophotonics; natural science methods in a study of cultural heritage items; metrology, standardization, and monitoring in nanotechnology.
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