Enhancing in vitro growth and development of Dalbergia nigra shoots using microporous membranes: impacts on gas exchange, protein profile, ethylene, and polyamine levels
IF 2.3 3区 生物学Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Renan Carrari-Santos, Rosana Gobbi Vettorazzi, Tadeu dos Reis Oliveira, Mateus Santana Rodrigues, Edinaldo de Oliveira Alves Sena, Jurandi Gonçalves de Oliveira, Vanildo Silveira, Claudete Santa-Catarina
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引用次数: 0
Abstract
The use of lids with microporous membranes can enhance in vitro plant growth by promoting gas exchange, including ethylene exchange. We aimed to evaluate the influence of the microporous membrane, explant type, and culture medium on the in vitro development of Dalbergia nigra shoots, as well as on the protein profile, polyamines (PAs), ethylene and CO2 levels. A total of 465 proteins were identified, of which 148 were differentially accumulated proteins, being 73 proteins up- and 75 down-accumulated in shoots grown in sealed-lid culture flasks containing microporous membranes compared to those grown without. The use of microporous membranes plays an important role in the accumulation of proteins related to photosynthetic activity process, such as ruBisCO_large domain-containing protein; ribulose bisphosphate carboxylase small chain; ribulose bisphosphate carboxylase small chain protein; and chlorophyll a-b binding protein, chloroplastic. Conversely, the decreased accumulation of proteins associated with amino acid synthesis linked to ethylene biosynthesis, such as aspartate aminotransferase, in shoots grown with microporous membranes was related to the lower level of ethylene than in shoots grown without membranes. The microporous membrane promoted an increase in total free PAs and putrescine contents in shoots, while higher CO2 levels were detected in shoots grown without a microporous membrane. This is the first study showing the effects of promoting gas exchange on shoot development in D. nigra, in addition to exploring the interaction of protein accumulation with ethylene. This research can improve the understanding of propagation systems in D. nigra, an endangered species.
期刊介绍:
This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues.
The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.