Research on the rapid tissue breeding technology of the endangered plant Cibotium barometz (L.) J. Sm.

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Qiaoling Yu, Wei Lin, Xingjian Yu, Ben Qin, Ying Liang, Fan Wei, Shuangshuang Qin, Kunhua Wei
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Abstract

Cibotium barometz (L.) J. Sm., a fern, holds significant value in traditional Chinese medicine. This research aimed to establish a rapid tissue breeding technology for Cibotium barometz (L.) and provide a theoretical basis for its artificial breeding and industrial planting. The optimum conditions for spores germination were selected, including both the best sterilization time and the ideal spore germination medium. Subsequently, the optimal medium for proliferation and differentiation were chosen, and the optimal concentration of plant hormones was screened to determine the optimal medium. The rooting experiment was performed in two single-factor experiments by using differentiated sporophytes as materials. These experiments showed that the optimal sterilization time was 11 min and the best medium for germination was 1/8MS + 0.02 mg/L IAA. MS medium was suitable for proliferation, while WPM medium was suitable for sporophyte differentiation. The best medium for growth was MS + 1.0 mg/L IAA + 0.5 mg/L 6-BA. In the absence of sucrose, the seedlings barely grew and failed to differentiate into sporophytes. They showed optimal growth and differentiation at a sucrose concentration of 20 g/L. The plant growth regulators plant growth regulators used, including IAA, NAA, 6-BA, and GA3, showed more effective differentiation, wherease KT had a slightly weaker effect. The rooting rate reached the maximum when the concentrations of ABT and IBA were at 1.5 mg•L− 1 and IAA was at 1.0 mg•L− 1. The IBA had a significant effect on the average and the longest root length, while the three PGRs had no obvious effect on the average or maximum diameter. Plant tissue culture seedlings still have good genetic stability after subsequent generation. In this study, the optimal cultivation conditions for rapid breeding technology in tissue culture, covering stages from germination and growth to differentiation and rooting, were obtained. Moreover, sporophytes were successfully transplanted to the external environment. This study provided a theoretical reference for the industrial planting of C. barometz.

Abstract Image

濒危植物 Cibotium barometz (L.) J. Sm.
蕨菜(Cibotium barometz (L.) J. Sm.)具有重要的中药价值。本研究旨在建立蕨类植物 Cibotium barometz (L.) 的快速组织育种技术,为其人工育种和工业化种植提供理论依据。研究选择了孢子萌发的最佳条件,包括最佳灭菌时间和理想的孢子萌发培养基。随后,选择了增殖和分化的最佳培养基,并对植物激素的最佳浓度进行了筛选,确定了最佳培养基。以分化的孢子体为材料,进行了两次单因素生根实验。实验结果表明,最佳灭菌时间为 11 分钟,最佳发芽培养基为 1/8MS + 0.02 mg/L IAA。MS 培养基适合增殖,而 WPM 培养基适合孢子体分化。最佳生长培养基为 MS + 1.0 mg/L IAA + 0.5 mg/L 6-BA。在没有蔗糖的情况下,幼苗几乎不生长,也不能分化成孢子体。当蔗糖浓度为 20 克/升时,秧苗的生长和分化达到最佳状态。所使用的植物生长调节剂(包括 IAA、NAA、6-BA 和 GA3)的分化效果较好,而 KT 的效果稍弱。当 ABT 和 IBA 的浓度为 1.5 mg-L- 1,IAA 的浓度为 1.0 mg-L- 1 时,生根率达到最大值。IBA 对平均根长和最长根长有明显影响,而三种 PGR 对平均直径和最大直径没有明显影响。植物组培苗在后续世代中仍具有良好的遗传稳定性。本研究获得了组织培养快速育种技术的最佳栽培条件,涵盖了从发芽、生长到分化和生根的各个阶段。此外,还成功地将孢子体移植到外部环境中。该研究为巴美茨的工业化种植提供了理论参考。
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来源期刊
Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物
CiteScore
5.40
自引率
13.30%
发文量
203
审稿时长
3.3 months
期刊介绍: This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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