Efficient plant regeneration through callus culture in Hedychium spicatum Buch.Ham. Ex. D. Don using response surface methodology

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Vibhash Dhyani, I. D. Bhatt, Veena Pande
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Abstract

Hedychium spicatum (Family-Zingiberaceae), commonly known as spiked ginger lily, is widely known for its medicinal properties and high market demand. The species is harvested mainly from the wild to meet the raw material requirements for the pharmaceutical and cosmaceutical industries; therefore, it needs urgent attention for its conservation and mass production. The present study developed an efficient in vitro propagation protocol for large-scale species production. The central composite design-response surface methodology (CCD-RSM) was designed to optimize the plant growth regulators (PGRs) concentration for maximum callus production, shoot regeneration, and rooting. The seed was used as explants in Murashige and Skoog (MS) medium with different concentrations of naphthalene acetic acid (NAA; 2.5-5.0 µM) in combination with thidiazuron (TDZ; 2.5-5.0 µM) for callus induction. TDZ (4.76 µM) with NAA (2.84 µM) showed maximum callus induction (100%) after 6 weeks of incubation. Callus pieces were transferred to MS medium with different concentrations of TDZ, NAA, and Indole-3-butyric acid (IBA) for shoot regeneration. The highest regeneration frequency (100%) was observed on MS medium enriched with TDZ (2.72 µM) and NAA (3.5 µM) that showed a maximum number of shoots/explants (16.34 no.). Regenerated shoots were rooted better (average number of roots/shoot − 11.75) on MS medium with NAA (2.11 µM) and IBA (1.17 µM) in combination. After the subsequent acclimatization and hardening process in the greenhouse, the plants were planted in the experimental field, and their survival rate was 89% after 2 years of establishment. The protocol established in the present study has prospects to meet the challenges of quality planting material for large-scale cultivation and raw material sources for industrial utilization.

Abstract Image

通过 Hedychium spicatum Buch.Ham. 的胼胝体培养实现高效植物再生Ex.D. Don 使用响应面方法
刺五加(Hedychium spicatum)(景天科),俗称刺五加,因其药用价值和高市场需求而广为人知。该物种主要从野外采集,以满足制药和化妆品行业对原材料的需求;因此,迫切需要对其进行保护和大规模生产。本研究开发了一种用于大规模物种生产的高效体外繁殖方案。设计了中央复合设计-响应面方法(CCD-RSM)来优化植物生长调节剂(PGRs)的浓度,以获得最大的胼胝体产量、芽再生和生根率。在含有不同浓度的萘乙酸(NAA;2.5-5.0 µM)和噻虫嗪(TDZ;2.5-5.0 µM)的 Murashige and Skoog(MS)培养基中,用种子作为外植体进行胼胝体诱导。培养 6 周后,TDZ(4.76 µM)与 NAA(2.84 µM)的胼胝体诱导率最高(100%)。将胼胝体转移到含有不同浓度 TDZ、NAA 和吲哚-3-丁酸(IBA)的 MS 培养基上进行芽再生。在富含 TDZ(2.72 µM)和 NAA(3.5 µM)的 MS 培养基上观察到的再生频率最高(100%),芽/外植体数量最多(16.34 个)。在含有 NAA(2.11 µM)和 IBA(1.17 µM)的 MS 培养基上,再生芽的生根情况更好(平均根数/芽为 11.75)。在温室中经过适应性培养和硬化处理后,这些植株被种植到实验田中,2 年后成活率为 89%。本研究制定的方案有望为大规模栽培提供优质的种植材料,并为工业利用提供原料来源。
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来源期刊
Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture 生物-生物工程与应用微生物
CiteScore
5.40
自引率
13.30%
发文量
203
审稿时长
3.3 months
期刊介绍: This journal highlights the myriad breakthrough technologies and discoveries in plant biology and biotechnology. Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. It examines the transcriptional and/or translational events involved in gene regulation as well as those molecular controls involved in morphogenesis of plant cells and tissues. The journal also covers practical and applied plant biotechnology, including regeneration, organogenesis and somatic embryogenesis, gene transfer, gene flow, secondary metabolites, metabolic engineering, and impact of transgene(s) dissemination into managed and unmanaged plant systems.
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