{"title":"Overexpression of PpGL2 from Prunus persica Enhanced Soybean Drought Tolerance","authors":"W. Li, L. Zhao, S. F. Jing, D. H. Li, H. Y. Li","doi":"10.1134/s1021443724605469","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The HD-ZIP transcription factor family plays crucial roles in plant growth and abiotic stress responses. While its diverse functions and regulatory mechanisms are well-documented, its role in conferring abiotic stress tolerance in peaches remains largely unexplored. Here, we report the bioinformatics profile of PpGL2, a member of the HD-ZIP transcription factor family, and its integration into the soybean genome to assess its potential impact on drought tolerance. Localization studies in onion cells revealed nuclear localization of PpGL2-GFP fusion protein, while yeast hybridization experiments demonstrated its transactivation and DNA binding abilities. <i>PpGL2</i> overexpression under drought conditions led to reduced accumulation of reactive oxygen species and malondialdehyde compared to wild-type, decreased rate of water loss, and increased chlorophyll and relative water content. Additionally, <i>PpGL2</i> overexpression promoted soybean height and root length under drought stress, accompanied by altered transcription levels of stress-related genes across different plant genotypes. Furthermore, <i>PpGL2</i> overexpression enhanced oxidative tolerance. Therefore, our findings suggest that <i>PpGL2</i> overexpression holds promise for enhancing soybean drought resistance, offering a novel approach to improving soybean drought resistance.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":"23 1","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1021443724605469","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The HD-ZIP transcription factor family plays crucial roles in plant growth and abiotic stress responses. While its diverse functions and regulatory mechanisms are well-documented, its role in conferring abiotic stress tolerance in peaches remains largely unexplored. Here, we report the bioinformatics profile of PpGL2, a member of the HD-ZIP transcription factor family, and its integration into the soybean genome to assess its potential impact on drought tolerance. Localization studies in onion cells revealed nuclear localization of PpGL2-GFP fusion protein, while yeast hybridization experiments demonstrated its transactivation and DNA binding abilities. PpGL2 overexpression under drought conditions led to reduced accumulation of reactive oxygen species and malondialdehyde compared to wild-type, decreased rate of water loss, and increased chlorophyll and relative water content. Additionally, PpGL2 overexpression promoted soybean height and root length under drought stress, accompanied by altered transcription levels of stress-related genes across different plant genotypes. Furthermore, PpGL2 overexpression enhanced oxidative tolerance. Therefore, our findings suggest that PpGL2 overexpression holds promise for enhancing soybean drought resistance, offering a novel approach to improving soybean drought resistance.
期刊介绍：
Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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