Alleviating Salt-Induced Effects in Stevia rebaudiana Via Exogenous Supply of Titanium Dioxide Nanoparticles and Putrescine

IF 1.1 4区 生物学 Q3 PLANT SCIENCES
M. Gerami, P. Majidian, M. Andarza, H. R. Ghorbani
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引用次数: 0

Abstract

Salinity poses a serious challenge for stevia cultivation to meet growing demand. This study investigates protective supplements for stevia grown under saline conditions. Seedlings were subjected to titanium dioxide nanoparticles (0, 150, and 300 ppm), putrescine (0 and 1 mM), and salt (0, 75, and 150 mM) in a factorial design. Morphological traits (plant height, leaf dry and fresh weight), photosynthetic pigments (chlorophyll a, b, total), proline content, soluble and glycoside sugars (rebaudioside A and stevioside), and antioxidant enzyme activity (catalase and peroxidase) were measured. Higher salt concentrations (150 mM NaCl) reduced plant height, leaf dry and fresh weight, and chlorophyll but increased proline, soluble and glycoside sugars, and antioxidant enzyme activity. Foliar applications of putrescine (1 mM) and TiO2 nanoparticles (150 and 300 ppm) mitigated salt stress by improving growth and physiological properties. Simultaneous application of putrescine (1 mM) and TiO2 nanoparticles (300 ppm) was most effective at enhancing secondary metabolite production, important for the pharmaceutical industry. This study demonstrates protective roles of putrescine and TiO2 nanoparticles for stevia cultivation under saline conditions.

Abstract Image

通过外源供应二氧化钛纳米颗粒和普陀碱减轻盐对甜叶菊的影响
摘要 盐碱地给甜叶菊种植带来了严峻挑战,难以满足日益增长的需求。本研究调查了在盐碱条件下种植甜叶菊的保护性补充剂。采用因子设计,将幼苗置于二氧化钛纳米颗粒(0、150 和 300 ppm)、腐胺(0 和 1 mM)以及盐(0、75 和 150 mM)的环境中。对植物的形态特征(株高、叶片干重和鲜重)、光合色素(叶绿素 a、b、总叶绿素)、脯氨酸含量、可溶性糖和糖苷(甜叶菊甙 A 和甜菊糖甙)以及抗氧化酶活性(过氧化氢酶和过氧化物酶)进行了测定。较高的盐浓度(150 mM NaCl)会降低植株高度、叶片干重和鲜重以及叶绿素,但会增加脯氨酸、可溶性糖和糖苷以及抗氧化酶的活性。叶面施用腐胺(1 mM)和 TiO2 纳米颗粒(150 和 300 ppm)可改善生长和生理特性,从而缓解盐胁迫。同时施用腐霉利(1 毫摩尔)和 TiO2 纳米颗粒(300 ppm)对提高次生代谢产物的产量最为有效,而次生代谢产物对制药业非常重要。本研究证明了盐碱条件下腐植酸和 TiO2 纳米粒子对甜叶菊栽培的保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.00
自引率
14.30%
发文量
107
审稿时长
6 months
期刊介绍: Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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