{"title":"A novel enzyme-assisted n-hexane/ethanol biphasic method to extract and separate heterotrophic Chlorella lipids for anti-inflammatory activity","authors":"","doi":"10.1016/j.algal.2024.103639","DOIUrl":null,"url":null,"abstract":"<div><p><em>Chlorella</em> lipids are rich in essential fatty acids and have potential for development in food and healthcare products. This study established a novel enzyme-assisted biphasic extraction to obtain functional lipids from <em>Chlorella</em> sp. MBFJNU-17 biomass, and evaluated the anti-inflammatory function of the extracted lipids. The obtained results showed that the enzyme-assisted biphasic extraction mediated with pectinase and organic solvents (<em>n</em>-hexane and ethanol) achieved 17.3 % <em>Chlorella</em> cell wall disruption and 268.30 mg/g lipid extraction rate. After the optimization of this process, the <em>n</em>-hexane phase had 83.20 % neutral lipids (NLs), the ethanol phase contained 94.02 % polar lipids (53.65 % glycolipids (GLs) and 40.37 % phospholipids (PLs)), manifesting that the established biphasic lipid extraction exhibited the attracting feature to separate the NLs and polar lipids after lipids extraction. In term of the anti-inflammatory, the <em>Chlorella</em> polar lipids were found to perform superior anti-inflammatory effect with lower levels of inflammatory factors TNF-α and IL-6 than those of NLs. Compared to the lipids in the <em>n</em>-hexane and ethanol phases, three individual lipids (NL, GLs and PLs) performed poorer anti-inflammatory effects, indicating that the synergistic action could be effectively improved by the combination of three lipids. Taken together, the developed enzyme-assisted biphasic extraction method for <em>Chlorella</em> sp. MBFJNU-17 lipids was the potential lipid extraction process to concurrently extract and separate anti-inflammatory lipids. The study offered essential and scientific details to exploit high-value <em>Chlorella</em> lipids for nutritional and healthy bioproducts.</p></div>","PeriodicalId":7855,"journal":{"name":"Algal Research-Biomass Biofuels and Bioproducts","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Algal Research-Biomass Biofuels and Bioproducts","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2211926424002510","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Chlorella lipids are rich in essential fatty acids and have potential for development in food and healthcare products. This study established a novel enzyme-assisted biphasic extraction to obtain functional lipids from Chlorella sp. MBFJNU-17 biomass, and evaluated the anti-inflammatory function of the extracted lipids. The obtained results showed that the enzyme-assisted biphasic extraction mediated with pectinase and organic solvents (n-hexane and ethanol) achieved 17.3 % Chlorella cell wall disruption and 268.30 mg/g lipid extraction rate. After the optimization of this process, the n-hexane phase had 83.20 % neutral lipids (NLs), the ethanol phase contained 94.02 % polar lipids (53.65 % glycolipids (GLs) and 40.37 % phospholipids (PLs)), manifesting that the established biphasic lipid extraction exhibited the attracting feature to separate the NLs and polar lipids after lipids extraction. In term of the anti-inflammatory, the Chlorella polar lipids were found to perform superior anti-inflammatory effect with lower levels of inflammatory factors TNF-α and IL-6 than those of NLs. Compared to the lipids in the n-hexane and ethanol phases, three individual lipids (NL, GLs and PLs) performed poorer anti-inflammatory effects, indicating that the synergistic action could be effectively improved by the combination of three lipids. Taken together, the developed enzyme-assisted biphasic extraction method for Chlorella sp. MBFJNU-17 lipids was the potential lipid extraction process to concurrently extract and separate anti-inflammatory lipids. The study offered essential and scientific details to exploit high-value Chlorella lipids for nutritional and healthy bioproducts.
期刊介绍:
Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment