{"title":"Rapid monitoring and early warning of Phaeocystis globosa bloom based on an effective electrochemical biosensor","authors":"","doi":"10.1016/j.bej.2024.109444","DOIUrl":null,"url":null,"abstract":"<div><p>Harmful algal blooms (HABs) have posed a significant threat to human society and the ecological environment. In particular, the outbreak of <em>Phaeocystis globosa</em> (<em>P. globosa</em>) bloom could affect coasts nuclear power safety. Unfortunately, current ecological monitoring tools fail to dynamically detect the densities of solitary cells from <em>P. globosa</em> in the pre-outbreak phases, thus affecting early interventions. In the study, an effective electrochemical DNA biosensor was developed to serve the rapid and effective detection of <em>P. globosa</em> DNA through a specific DNA probe strategy. Especially, its good specificity and lower limit of detection (LOD, 17 pg/μL or 1063 cells/L) met the monitoring requirement of solitary-cell population change of <em>P. globosa</em> before the cyst formation (threshold: 1.0 × 10<sup>7</sup> cells/L), which is the key step in the algal bloom outbreaks and influences the outbreak cycle and scale. Furthermore, the accuracy of this electrochemical biosensor for the quantitative detection of solitary-cell <em>P. globosa</em> was confirmed by using the classical microscopic examination techniques (r = 0.981, <em>P</em> < 0.001). Moreover, its applicability was also validated by actual sample testing (r = 0.996, <em>P</em> < 0.001). Therefore, the novel technology offers great potential to improve dynamic detection and early warning of <em>P. globosa</em> bloom.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X24002316","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Harmful algal blooms (HABs) have posed a significant threat to human society and the ecological environment. In particular, the outbreak of Phaeocystis globosa (P. globosa) bloom could affect coasts nuclear power safety. Unfortunately, current ecological monitoring tools fail to dynamically detect the densities of solitary cells from P. globosa in the pre-outbreak phases, thus affecting early interventions. In the study, an effective electrochemical DNA biosensor was developed to serve the rapid and effective detection of P. globosa DNA through a specific DNA probe strategy. Especially, its good specificity and lower limit of detection (LOD, 17 pg/μL or 1063 cells/L) met the monitoring requirement of solitary-cell population change of P. globosa before the cyst formation (threshold: 1.0 × 107 cells/L), which is the key step in the algal bloom outbreaks and influences the outbreak cycle and scale. Furthermore, the accuracy of this electrochemical biosensor for the quantitative detection of solitary-cell P. globosa was confirmed by using the classical microscopic examination techniques (r = 0.981, P < 0.001). Moreover, its applicability was also validated by actual sample testing (r = 0.996, P < 0.001). Therefore, the novel technology offers great potential to improve dynamic detection and early warning of P. globosa bloom.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics
Biosensors and Biodevices including biofabrication and novel fuel cell development
Bioseparations including scale-up and protein refolding/renaturation
Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
Bioreactor Systems including characterization, optimization and scale-up
Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release
Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites
Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation
Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
Protein Engineering including enzyme engineering and directed evolution.