{"title":"Optimized Protocol for Production and Extraction of Colanic Acid from E. coli Culture","authors":"S. A. Tsvetikova, E. I. Koshel","doi":"10.1134/S1068162024040344","DOIUrl":null,"url":null,"abstract":"<p><b>Objective:</b> Colanic acid (CA) is a promising and actively studied polysaccharide, which geroprotective and stimulating properties have already been described in animal models. Different protocols are used to isolate and purify CA in research, some of which may result in contamination with proteins, DNA, and other polysaccharides. <b>Methods:</b> In order to effectively further investigate the biological properties of CA, we developed 5 protocols for CA isolation, differed in cultivation condition, protein precipitation, and polysaccharide precipitants. <b>Results and Discussion:</b> We found that the most suitable protocol for CA isolation includes protein removal with trichloroacetic acid and polysaccharide precipitation with ethanol (95%, 3 volumes). <b>Conclusions:</b> Such a simple protocol will serve to standardize CA studies and can be applied to obtain large volumes of polysaccharide.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1134/S1068162024040344","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: Colanic acid (CA) is a promising and actively studied polysaccharide, which geroprotective and stimulating properties have already been described in animal models. Different protocols are used to isolate and purify CA in research, some of which may result in contamination with proteins, DNA, and other polysaccharides. Methods: In order to effectively further investigate the biological properties of CA, we developed 5 protocols for CA isolation, differed in cultivation condition, protein precipitation, and polysaccharide precipitants. Results and Discussion: We found that the most suitable protocol for CA isolation includes protein removal with trichloroacetic acid and polysaccharide precipitation with ethanol (95%, 3 volumes). Conclusions: Such a simple protocol will serve to standardize CA studies and can be applied to obtain large volumes of polysaccharide.
摘要目的：可乐定酸（CA）是一种前景广阔、研究活跃的多糖，已在动物模型中描述了其老年保护和刺激特性。研究中使用了不同的方案来分离和纯化 CA，其中一些方案可能会导致蛋白质、DNA 和其他多糖的污染。方法：为了有效地进一步研究CA的生物学特性，我们开发了5种分离CA的方案，它们在培养条件、蛋白质沉淀和多糖沉淀剂方面各不相同。结果与讨论我们发现最适合的 CA 分离方案包括用三氯乙酸去除蛋白质和用乙醇（95%，3 体积）沉淀多糖。得出结论：这种简单的方案将有助于实现 CA 研究的标准化，并可用于获取大量多糖。
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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