Development and Application of Novel Extraction Method to Analyze Herbal Supplements and Adulterant Determination by GCxGC/Q-TOFMS

IF 2.6 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2024-08-06 DOI:10.1007/s12161-024-02657-y

Jhonatan Bispo de Oliveira, Maria Fernanda Mendes Carvalho, Patterson Patrício de Souza, Zenilda de Lourdes Cardeal

{"title":"Development and Application of Novel Extraction Method to Analyze Herbal Supplements and Adulterant Determination by GCxGC/Q-TOFMS","authors":"Jhonatan Bispo de Oliveira, Maria Fernanda Mendes Carvalho, Patterson Patrício de Souza, Zenilda de Lourdes Cardeal","doi":"10.1007/s12161-024-02657-y","DOIUrl":null,"url":null,"abstract":"<div><p>This manuscript proposes a new extraction method for analyzing natural compounds in herbal supplements for weight loss and determining adulterants by a comprehensive two-dimensional gas chromatography system coupled to a quadrupole time-of-flight mass spectrometer (GCxGC/Q-TOFMS). In this new approach, a hydrophilic microporous cartridge (HMCart) was developed to enclose the samples, protecting the SPME (solid-phase microextraction) fibers. The cartridge is loaded with herbal supplement samples during direct immersion of SPME fiber in the aqueous phase to allow the analytes to be trapped. The new sample preparation method using chromatographic analyses enabled the detection of 650 compounds, including natural compounds, such as terpenes, aldehydes, ketones, and fatty acids. Adulterants found in other studies in the literature, such as sibutramine and ephedrine. The fluoxetine and caffeine were detected and quantified. The new method has achieved the goal of simple, effective, solvent-free, and versatile sample preparation that can be applied in future studies, including determining other compound classes in various matrix samples.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 9","pages":"1267 - 1280"},"PeriodicalIF":2.6000,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Analytical Methods","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s12161-024-02657-y","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

This manuscript proposes a new extraction method for analyzing natural compounds in herbal supplements for weight loss and determining adulterants by a comprehensive two-dimensional gas chromatography system coupled to a quadrupole time-of-flight mass spectrometer (GCxGC/Q-TOFMS). In this new approach, a hydrophilic microporous cartridge (HMCart) was developed to enclose the samples, protecting the SPME (solid-phase microextraction) fibers. The cartridge is loaded with herbal supplement samples during direct immersion of SPME fiber in the aqueous phase to allow the analytes to be trapped. The new sample preparation method using chromatographic analyses enabled the detection of 650 compounds, including natural compounds, such as terpenes, aldehydes, ketones, and fatty acids. Adulterants found in other studies in the literature, such as sibutramine and ephedrine. The fluoxetine and caffeine were detected and quantified. The new method has achieved the goal of simple, effective, solvent-free, and versatile sample preparation that can be applied in future studies, including determining other compound classes in various matrix samples.

Abstract Image

查看原文本刊更多论文

利用 GCxGC/Q-TOFMS 开发和应用新型萃取方法分析草药补充剂并测定掺假物质

本手稿提出了一种新的萃取方法，通过与四极杆飞行时间质谱仪（GCxGC/Q-TOFMS）联用的综合二维气相色谱系统，分析草药减肥保健品中的天然化合物并确定掺杂物。在这种新方法中，开发了一种亲水性微孔滤筒（HMCart）来封装样品，保护 SPME（固相微萃取）纤维。在将 SPME 纤维直接浸入水相时，将草药补充剂样品装入滤芯，使分析物被截留。使用色谱分析的新样品制备方法能够检测出 650 种化合物，包括萜烯、醛、酮和脂肪酸等天然化合物。文献中其他研究发现的掺杂物，如西布曲明和麻黄碱。对氟西汀和咖啡因进行了检测和定量。新方法实现了简单、有效、无溶剂和多功能样品制备的目标，可用于今后的研究，包括测定各种基质样品中的其他化合物类别。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.