Reducing fluorescence interference for improved Raman spectroscopic analysis of plant cell walls

IF 3.1 2区农林科学 Q1 FORESTRY

Wood Science and Technology Pub Date : 2024-08-08 DOI:10.1007/s00226-024-01587-6

Maozhi Zhang, Yun Feng, Li Li, Xun Zhang, Feng Xu

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Abstract

Lignin fluorescence in plant cell walls significantly interferes with Raman spectroscopic signals, resulting in compromised analytical accuracy and resolution. To address this issue, a strategy was implemented to both reduce the absolute lignin content in samples and prepare thinner plant tissue sections. This approach involved embedding plant samples in LR White resin, complemented by an ultrathin sectioning technique. Additionally, algorithms were developed to eliminate the impact of resin spectra on the imaging process. These advancements collectively enhanced the performance of Raman spectroscopy by effectively diminishing the disruptive effects of lignin fluorescence. Further analysis with confocal laser scanning microscopy (CLSM) elucidated the presence of aggregation-induced luminescence (AIE) in plant tissues, revealing a direct correlation with lignin concentration. These findings not only offer a new perspective for the application of Raman spectroscopy in plant science, but also pave the way for advancements in tip-enhanced Raman spectroscopy (TERS) detection.

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减少荧光干扰，改进植物细胞壁的拉曼光谱分析

植物细胞壁中的木质素荧光会严重干扰拉曼光谱信号，从而影响分析精度和分辨率。为了解决这个问题，我们采用了一种策略，既能降低样品中木质素的绝对含量，又能制备更薄的植物组织切片。这种方法包括将植物样本嵌入 LR White 树脂中，并辅以超薄切片技术。此外，还开发了算法来消除树脂光谱对成像过程的影响。这些先进技术共同提高了拉曼光谱的性能，有效降低了木质素荧光的破坏性影响。利用共焦激光扫描显微镜（CLSM）进行的进一步分析阐明了植物组织中存在聚集诱导发光（AIE），揭示了与木质素浓度的直接相关性。这些发现不仅为拉曼光谱在植物科学中的应用提供了新的视角，还为尖端增强拉曼光谱（TERS）检测技术的发展铺平了道路。

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来源期刊

Wood Science and Technology 工程技术-材料科学：纸与木材

CiteScore

5.90

自引率

5.90%

发文量

审稿时长

3 months

期刊介绍： Wood Science and Technology publishes original scientific research results and review papers covering the entire field of wood material science, wood components and wood based products. Subjects are wood biology and wood quality, wood physics and physical technologies, wood chemistry and chemical technologies. Latest advances in areas such as cell wall and wood formation; structural and chemical composition of wood and wood composites and their property relations; physical, mechanical and chemical characterization and relevant methodological developments, and microbiological degradation of wood and wood based products are reported. Topics related to wood technology include machining, gluing, and finishing, composite technology, wood modification, wood mechanics, creep and rheology, and the conversion of wood into pulp and biorefinery products.