Unraveling defense strategies: gene expression dynamics in blast-resistant vs. susceptible aromatic rice landraces

IF 0.9 4区农林科学 Q4 PLANT SCIENCES

Australasian Plant Pathology Pub Date : 2024-08-10 DOI:10.1007/s13313-024-00983-7

U. Keerthana, A. K. Senapati, Manas K. Bag, S. R. Prabhukarthikeyan, Shraddha B. Sawant, M. K. Mishra, S. D. Mohapatra

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Abstract

In this study, efforts were made to understand the defence responses in resistant and susceptible aromatic landraces against the rice leaf blast pathogen, Magnaporthe oryzae. Four resistant genotypes (Benugopal, Manas, Karpurkali, and Kalazeera) and two susceptible genotypes (Kalikati 2 and Kalikati 1) underwent biochemical analysis in relation to blast disease. The results of the biochemical analysis revealed increased enzyme activity levels, namely peroxidase, phenylalanine ammonia-lyase, polyphenol oxidase, superoxide dismutase, and catalase, in the resistant genotypes (Benugopal (AC44184) and Karpurkali (AC44155)) in comparison to the susceptible ones [Kalikati 2 (AC44236) and Kalikati 1 (AC44156)]. The same trend has been seen in the assays for β-1,3-glucanase and total phenols. RT-qPCR evaluation has been performed to establish the expression levels of PO, PAL, PPO, SOD, and CAT in the four resistant genotypes and the two susceptible ones after inoculation with M. oryzae (RLB 06) at various time intervals. Noteworthy variations in defence gene expression were noted among the genotypes. The expression of defence genes was upregulated in the resistant genotypes [Benugopal (AC44184) and Karpurkali (AC44155)] compared to their susceptible ones. Gene expression studies result indicated that the relative defence genes expression levels related to the spectrophotometric data.

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揭示防御策略：抗稻瘟病与易感香稻品种的基因表达动态变化

本研究旨在了解抗性和易感性芳香陆稻品种对稻叶瘟病原体 Magnaporthe oryzae 的防御反应。对四个抗病基因型（Benugopal、Manas、Karpurkali 和 Kalazeera）和两个易感基因型（Kalikati 2 和 Kalikati 1）进行了与稻瘟病有关的生化分析。生化分析结果显示，与易感基因型[Kalikati 2 (AC44236) 和 Kalikati 1 (AC44156)]相比，抗病基因型（Benugopal (AC44184) 和 Karpurkali (AC44155)）的过氧化物酶、苯丙氨酸氨化酶、多酚氧化酶、超氧化物歧化酶和过氧化氢酶的酶活性水平有所提高。β-1,3-葡聚糖酶和总酚的检测也呈现出同样的趋势。对四种抗性基因型和两种易感基因型在不同时间间隔接种 M. oryzae (RLB 06) 后的 PO、PAL、PPO、SOD 和 CAT 表达水平进行了 RT-qPCR 评估。不同基因型的防御基因表达存在显著差异。与抗性基因型[Benugopal（AC44184）和 Karpurkali（AC44155）]相比，抗性基因型[Benugopal（AC44184）和 Karpurkali（AC44155）]的防卫基因表达上调。基因表达研究结果表明，相对防御基因表达水平与分光光度数据有关。

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来源期刊

Australasian Plant Pathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Australasian Plant Pathology presents new and significant research in all facets of the field of plant pathology. Dedicated to a worldwide readership, the journal focuses on research in the Australasian region, including Australia, New Zealand and Papua New Guinea, as well as the Indian, Pacific regions. Australasian Plant Pathology is the official journal of the Australasian Plant Pathology Society.