Volatile organic compounds (VOCs) of Bacillus velezensis TA-1 exhibit toxic effects against Meloidogyne incognita

IF 4.3 1区 农林科学 Q1 ENTOMOLOGY
Xiaoxue Ji, Miao Fan, Di Wang, Siqi Zhang, Shouan Zhang, Yong Liu, Kang Qiao
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Abstract

The use of Bacillus volatiles to manage root-knot nematodes (RKNs) is a topic that gained extensive attention because it is safe and environmentally benign. Bacillus velezensis TA-1, isolated from a continuously cropped soil, exhibited strong nematicidal activity against RKNs in vitro and in field conditions. However, it remains poorly understood whether TA-1 volatile organic compounds (VOCs) could be effective against Meloidogyne incognita. In this study, the results of chemotaxis activity test revealed that B. velezensis TA-1 exhibited strong repellent effects on second-stage juveniles (J2s) of M. incognita. In in vitro assays, TA-1 VOCs in a three-compartment Petri dish assay exhibited a mortality rate of 84.1% at 48 h and 92.8% at 72 h to M. incognita J2s. Further evidence revealed that TA-1 VOCs significantly inhibited the gene expression of mpk-1, flp-18 and ord-1 by 61.0%, 44.8% and 54.5%, respectively. Moreover, TA-1 VOCs increased the content of reactive oxygen species (ROS), resulting in severe oxidative stress and death of nematodes. Results from a double-layered greenhouse experiment indicated that the populations of J2s in the soil and in cucumber roots were notably reduced by TA-1 VOCs compared to the untreated control. Furthermore, eleven VOCs were identified from TA-1 by solid-phase microextraction gas chromatography-mass spectrometry (SPME-GC/MS) analysis, of which benzaldehyde, 2-decanol, and 2-undecanone had strong contact nematicidal activities against M. incognita J2s. In addition, benzaldehyde and 2-dodecanone exhibited fumigation effects on M. incognita J2s. Overall, these results demonstrated that TA-1 VOCs exhibited direct contact nematicidal and fumigation activities against RKNs and could be considered as potential biocontrol agents for the control of RKNs.

Abstract Image

枯草芽孢杆菌 TA-1 的挥发性有机化合物 (VOC) 对黑僵菌具有毒性作用
利用挥发性芽孢杆菌治理根结线虫(RKNs)是一个受到广泛关注的课题,因为它安全且对环境无害。从连续耕作的土壤中分离出的枯草芽孢杆菌(Bacillus velezensis TA-1)在体外和田间条件下对根结线虫具有很强的杀线虫活性。然而,人们对 TA-1 挥发性有机化合物(VOCs)是否能有效杀灭黑线蝇仍知之甚少。在这项研究中,趋化活性测试结果表明,B. velezensis TA-1 对褐飞虱第二阶段幼虫(J2s)具有很强的驱避作用。在体外试验中,在三室培养皿试验中,TA-1 VOCs 在 48 小时内对 M. incognita J2s 的死亡率为 84.1%,72 小时内为 92.8%。进一步的证据表明,TA-1 VOCs 能显著抑制 mpk-1、flp-18 和 ord-1 的基因表达,抑制率分别为 61.0%、44.8% 和 54.5%。此外,TA-1 VOCs 还增加了活性氧(ROS)的含量,导致严重的氧化应激和线虫死亡。双层温室实验结果表明,与未处理的对照组相比,TA-1 VOCs 能显著减少土壤中和黄瓜根部的 J2s 数量。此外,通过固相微萃取气相色谱-质谱联用仪(SPME-GC/MS)分析,从 TA-1 中鉴定出 11 种挥发性有机化合物,其中苯甲醛、2-癸醇和 2-十一酮对 M. incognita J2s 具有很强的接触杀线虫活性。此外,苯甲醛和 2-十二酮对 M. incognita J2s 也有熏蒸作用。总之,这些结果表明,TA-1 VOCs 对 RKNs 具有直接接触杀线虫和熏蒸活性,可被视为控制 RKNs 的潜在生物控制剂。
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来源期刊
Journal of Pest Science
Journal of Pest Science 生物-昆虫学
CiteScore
10.40
自引率
8.30%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues. Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates. Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management. Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.
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