Intraocular fatty acids induce reinforcement of barrier functions on the outer blood-retinal barrier

IF 3
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引用次数: 0

Abstract

The aim of the present study was to elucidate unknown effects of intraocular fatty acids (ioFAs) including palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), arachidonic acid (C20:4), eicosapentaenoic acid (EPA, C20:5) and docosahexaenoic acid (DHA, C22:6) on the outer blood-retinal barrier (oBRB). For this purpose, human retinal pigment epithelium cell line ARPE19 was subjected to analyses for evaluating the following biological phenotypes: (1) cell viability, (2) cellular metabolic functions, (3) barrier functions by trans-epithelial electrical resistance (TEER), and (4) expression of tight junction (TJ) molecules. In the presence of 100 nM ioFAs, no significant effects on cell viability of ARPE19 cells was observed. While treatment with EPA or DHA tended to reduce non-mitochondrial oxygen consumption, most indices in mitochondrial functions were not markedly affected by treatment with ioFAs in ARPE19 cells. On the other hand, ioFAs except for palmitic acid and stearic acid significantly increased basal extracellular acidification rates, suggesting activated glycolysis or increased lactate production. Interestingly, TEER values of planar ARPE19 monolayer were significantly increased by treatment any ioFAs. Consistently, gene expression levels of TJ proteins were increased by treatment with ioFAs. Collectively, the findings presented herein suggest that ioFAs may contribute to reinforcement of barrier functions of the oBRB albeit there are some differences in biological effects depending on the type of ioFAs.

眼内脂肪酸可诱导强化外层血液-视网膜屏障的屏障功能。
本研究旨在阐明眼内脂肪酸(ioFAs)的未知影响,包括棕榈酸(C16:0)、硬脂酸(C18:0)、油酸(C18:1)、亚油酸(C18:2)、花生四烯酸(C20:4)、二十碳五烯酸(EPA,C20:5)和二十二碳六烯酸(DHA,C22:6)。为此,对人类视网膜色素上皮细胞系 ARPE19 进行了分析,以评估以下生物表型:(1) 细胞活力;(2) 细胞代谢功能;(3) 通过跨上皮电阻(TEER)评估屏障功能;(4) 紧密连接(TJ)分子的表达。在 100 nM ioFAs 存在的情况下,ARPE19 细胞的细胞活力没有受到明显影响。虽然用 EPA 或 DHA 处理往往会降低非线粒体耗氧量,但线粒体功能的大多数指标并没有受到用 ioFAs 处理 ARPE19 细胞的明显影响。另一方面,除棕榈酸和硬脂酸外,其他 ioFAs 会显著增加基础细胞外酸化率,这表明糖酵解被激活或乳酸生成增加。有趣的是,ARPE19 单层平面的 TEER 值在任何 ioFAs 的处理下都会明显增加。同样,TJ 蛋白的基因表达水平也因 ioFAs 的处理而增加。总之,本文的研究结果表明,ioFAs 可能有助于增强 oBRB 的屏障功能,尽管不同类型的 ioFAs 在生物效应方面存在一些差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Prostaglandins, leukotrienes, and essential fatty acids
Prostaglandins, leukotrienes, and essential fatty acids Clinical Biochemistry, Endocrinology, Diabetes and Metabolism
CiteScore
5.30
自引率
0.00%
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0
审稿时长
64 days
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