WRKY75 regulates anthocyanin accumulation in juvenile citrus tissues.

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2024-08-09 eCollection Date: 2024-08-01 DOI:10.1007/s11032-024-01490-9

Zhihao Lu, Jiaxian He, Jialing Fu, Yuping Huang, Xia Wang

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Abstract

The anthocyanin accumulation in juvenile tissues can enhance the ornamental value, attract pollinators, and help improve abiotic stress. Although transcriptional regulation studies of anthocyanin have been relatively extensive, there are few reports on the mechanism of anthocyanin accumulation in young tissues. This study reveals that many juvenile citrus tissues (flowers, leaves, and pericarp) undergo transient accumulation of anthocyanins, exhibiting a red coloration. Using weighted gene co-expression network analysis (WGCNA) identified CitWRKY75 as a candidate gene. After detecting the expression levels of CitWRKY75 in various citrus juvenile tissues, the expression trend of CitWRKY75 was highly consistent with the red exhibiting and fading. Overexpression of CitWRKY75 in tobacco significantly increased the anthocyanin content. LUC and yeast one-hybrid assay demonstrated that CitWRKY75 could bind to the promoter of CitRuby1(encoding the key transcription factor promoting anthocyanin accumulation) and promote its expression. Finally, comparing the expression levels of CitWRKY75 and CitRuby1 in the late development stage of blood orange found that CitWRKY75 was not the main regulatory factor for anthocyanin accumulation in the later stage. This study used reverse genetics to identify a transcription factor, CitWRKY75, upstream of CitRuby1, which promotes anthocyanin accumulation in citrus juvenile tissues.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01490-9.

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WRKY75 可调控柑橘幼果组织中花青素的积累。

幼苗组织中花青素的积累可以提高观赏价值，吸引传粉昆虫，并有助于改善非生物胁迫。虽然花青素的转录调控研究已经比较广泛，但有关幼年组织花青素积累机制的报道却很少。本研究揭示了许多柑橘幼果组织（花、叶和果皮）中花青素的短暂积累，并呈现出红色。通过加权基因共表达网络分析（WGCNA），发现 CitWRKY75 是一个候选基因。通过检测 CitWRKY75 在柑橘不同幼果组织中的表达水平，CitWRKY75 的表达趋势与红色的显现和消退高度一致。在烟草中过表达 CitWRKY75 能显著提高花青素含量。LUC和酵母单杂交实验证明，CitWRKY75能与CitRuby1（编码促进花青素积累的关键转录因子）的启动子结合并促进其表达。最后，比较血橙发育后期 CitWRKY75 和 CitRuby1 的表达水平发现，CitWRKY75 并不是后期花青素积累的主要调控因子。本研究利用反向遗传学鉴定了CitRuby1上游的转录因子CitWRKY75，该转录因子可促进柑橘幼果组织中花青素的积累：在线版本包含补充材料，可查阅 10.1007/s11032-024-01490-9。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.