New potential diagnostic markers for verrucous hyperplasia and verrucous carcinoma based on RNA-sequencing data

IF 2.3 3区生物学 Q3 BIOCHEMICAL RESEARCH METHODS

Molecular and Cellular Probes Pub Date : 2024-08-12 DOI:10.1016/j.mcp.2024.101980

Janghyun Kim , Jee-hye Kang , Myung-Giun Noh , Bora Lee , Yoo-Duk Choi , Ok Joon Kim , Young Kim

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引用次数: 0

Abstract

Verrucous carcinoma (VC) is a rare subtype of squamous cell carcinoma (SCC) characterized by its histological presentation as a low-grade tumor with no potential for metastasis, setting it apart from invasive SCC. However, distinguishing VC from its benign counterpart, verrucous hyperplasia (VH), is challenging due to their clinical and morphological similarities. Despite the importance of accurate diagnosis for determining treatment strategies, diagnosis for of VH and VC relied only on lesion recurrence after resection. To address this challenge, we generated RNA profiling data from tissue samples of VH and VC patients to identify novel diagnostic markers. We analyzed differentially expressed (DE) mRNA and long non-coding RNA (lncRNA) in tissue samples from VH and VC patients. Additionally, ChIP-X Enrichment Analysis 3 (ChEA3) was conducted to identify the top five transcription factors potentially regulating the expression of DE mRNAs in VH and VC. Our analysis of mRNA and lncRNA expression profiles in VH and VC provides insights into the underlying molecular characteristics of these diseases and offers potential new diagnostic markers. The identification of specific DE genes and lncRNAs may enable clinicians to more accurately differentiate between VH and VC, leading to better treatment choices.

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基于 RNA 序列数据的疣状增生和疣状癌潜在诊断新标记。

疣状癌（VC）是鳞状细胞癌（SCC）的一种罕见亚型，其特点是组织学表现为低级别肿瘤，没有转移的可能性，因此有别于浸润性 SCC。然而，由于疣状增生症（VH）在临床和形态上的相似性，将其与良性疣状增生症区分开来非常困难。尽管准确诊断对确定治疗策略非常重要，但对 VH 和 VC 的诊断仅依赖于切除后病灶的复发。为了应对这一挑战，我们从 VH 和 VC 患者的组织样本中获取了 RNA 图谱数据，以确定新的诊断标记物。我们分析了 VH 和 VC 患者组织样本中差异表达（DE）的 mRNA 和长非编码 RNA（lncRNA）。此外，我们还进行了 ChIP-X Enrichment Analysis 3（ChEA3）分析，以确定可能调控 VH 和 VC 中 DE mRNA 表达的五大转录因子。我们对VH和VC中mRNA和lncRNA表达谱的分析深入揭示了这些疾病的潜在分子特征，并提供了潜在的新诊断标记物。特异性 DE 基因和 lncRNA 的鉴定可使临床医生更准确地区分 VH 和 VC，从而做出更好的治疗选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular and Cellular Probes 生物-生化研究方法

CiteScore

6.80

自引率

0.00%

发文量

审稿时长

16 days

期刊介绍： MCP - Advancing biology throughâ€“omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.