In vivo CRISPR-Cas9 expression in Candida glabrata, Candida bracarensis, and Candida nivariensis: A versatile tool to study chromosomal break repair.

IF 2.2 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yeast Pub Date : 2024-09-01 Epub Date: 2024-08-09 DOI:10.1002/yea.3976
Killian Métivier, Youfang Zhou-Li, Cécile Fairhead
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引用次数: 0

Abstract

The CRISPR-Cas9 system is extremely useful for genome editing in many species, including the model yeast Saccharomyces cerevisiae, and other yeast species. We have previously reported the use of an inducible CRISPR-Cas9 system in Candida glabrata, which allows genome editing but also the study of double-strand break (DSB) repair. We report, in this study, a comparable system for C. glabrata, relying on a new plasmid, which is more stable than the previous one. We also report the use of this plasmid to induce DSBs in two additional human pathogens, Candida bracarensis and Candida nivariensis. We examine lethality induced by an in vivo DSB in the three species and describe the different types of nonhomologous end-joining (NHEJ) events detected in these three pathogens.

CRISPR-Cas9在格拉布氏念珠菌、布拉卡氏念珠菌和尼瓦瑞氏念珠菌中的体内表达:研究染色体断裂修复的多功能工具。
CRISPR-Cas9 系统对许多物种的基因组编辑非常有用,包括模式酵母酿酒酵母和其他酵母物种。我们以前曾报道过在念珠菌中使用诱导型 CRISPR-Cas9 系统的情况,该系统不仅能进行基因组编辑,还能研究双链断裂(DSB)修复。在本研究中,我们报告了一种可用于草履虫的类似系统,它依赖于一种新的质粒,这种质粒比以前的质粒更稳定。我们还报告了利用这种质粒诱导另外两种人类病原体--布拉卡氏念珠菌和尼瓦里念珠菌--DSB的情况。我们研究了这三种病原体体内DSB诱导的致死率,并描述了在这三种病原体中检测到的不同类型的非同源末端连接(NHEJ)事件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Yeast
Yeast 生物-生化与分子生物学
CiteScore
5.30
自引率
3.80%
发文量
55
审稿时长
3 months
期刊介绍: Yeast publishes original articles and reviews on the most significant developments of research with unicellular fungi, including innovative methods of broad applicability. It is essential reading for those wishing to keep up to date with this rapidly moving field of yeast biology. Topics covered include: biochemistry and molecular biology; biodiversity and taxonomy; biotechnology; cell and developmental biology; ecology and evolution; genetics and genomics; metabolism and physiology; pathobiology; synthetic and systems biology; tools and resources
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