Exploring modes of microbial interactions with implications for methane cycling.

IF 3.5 3区 生物学 Q2 MICROBIOLOGY
Kristof Brenzinger, Timo Glatter, Anna Hakobyan, Marion Meima-Franke, Hans Zweers, Werner Liesack, Paul L E Bodelier
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引用次数: 0

Abstract

Methanotrophs are the sole biological sink of methane. Volatile organic compounds (VOCs) produced by heterotrophic bacteria have been demonstrated to be a potential modulating factor of methane consumption. Here, we identify and disentangle the impact of the volatolome of heterotrophic bacteria on the methanotroph activity and proteome, using Methylomonas as model organism. Our study unambiguously shows how methanotrophy can be influenced by other organisms without direct physical contact. This influence is mediated by VOCs (e.g. dimethyl-polysulphides) or/and CO2 emitted during respiration, which can inhibit growth and methane uptake of the methanotroph, while other VOCs had a stimulating effect on methanotroph activity. Depending on whether the methanotroph was exposed to the volatolome of the heterotroph or to CO2, proteomics revealed differential protein expression patterns with the soluble methane monooxygenase being the most affected enzyme. The interaction between methanotrophs and heterotrophs can have strong positive or negative effects on methane consumption, depending on the species interacting with the methanotroph. We identified potential VOCs involved in the inhibition while positive effects may be triggered by CO2 released by heterotrophic respiration. Our experimental proof of methanotroph-heterotroph interactions clearly calls for detailed research into strategies on how to mitigate methane emissions.

探索微生物相互作用模式对甲烷循环的影响。
养甲烷菌是甲烷的唯一生物汇。异养细菌产生的挥发性有机化合物(VOCs)已被证明是甲烷消耗的潜在调节因素。在这里,我们以甲基单胞菌为模式生物,识别并厘清了异养细菌的挥发性有机化合物对甲烷营养体活性和蛋白质组的影响。我们的研究明确显示了甲烷营养如何在没有直接物理接触的情况下受到其他生物的影响。这种影响由挥发性有机化合物(如二甲基多硫化物)或/和呼吸过程中排放的二氧化碳介导,它们可以抑制甲烷营养体的生长和甲烷吸收,而其他挥发性有机化合物则对甲烷营养体的活性有刺激作用。根据甲烷营养体暴露于异养生物的挥发物还是二氧化碳,蛋白质组学发现了不同的蛋白质表达模式,其中受影响最大的酶是可溶性甲烷单加氧酶。甲烷营养体与异养生物之间的相互作用会对甲烷消耗产生强烈的积极或消极影响,这取决于与甲烷营养体相互作用的物种。我们发现潜在的挥发性有机化合物参与了抑制作用,而正效应可能是由异养生物呼吸释放的二氧化碳引发的。我们对甲烷营养体与异养生物相互作用的实验证明,显然需要对如何减少甲烷排放的策略进行详细研究。
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来源期刊
FEMS microbiology ecology
FEMS microbiology ecology 生物-微生物学
CiteScore
7.50
自引率
2.40%
发文量
132
审稿时长
3 months
期刊介绍: FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms
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