Effect of cryoprotectant and concentration on the sperm quality of walking catfish, Clarias batrachus, post-cryopreservation.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2024-09-01

L Handayani, S Maulida, S Rahayu, N M Razi, M Kocabas, F K Kocabas, M Wilkes, M N Siti-Azizah, K Eriani, N Fadli, Z A Muchlisin

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引用次数: 0

Abstract

Background: Walking catfish, Clarias batrachus is one of the native and most popular freshwater catfish species in Indonesia. However, cultivation faces challenges, particularly due to the scarcity of larvae resulting from underdeveloped breeding technologies. Cryopreservation is a method of storing sperm to maintain viability for a long period and support the breeding technology of the fish. Cryoprotectant, in this context, plays an important role in determining the success of sperm cryopreservation.

Objective: To determine the best type and concentration of cryoprotectant for cryopreservation of walking catfish sperm.

Materials and methods: A total of five different types of cryoprotectants, namely DMSO, glycerol, ethyl glycol, ethanol, and methanol, were tested at four concentration levels namely 0%, 5%, 10%, 15%, and 20%, each with four replications.

Results: The type and concentration of cryoprotectant had a significant effect on sperm motility and viability (P < 0.05). The best outcomes were obtained with 5% DMSO and ethyl glycol, 10% glycerol and methanol, as well as 15% ethanol.

Conclusion: The highest motility and viability values were obtained with 5% DMSO, resulting in its recommendation for cryopreservation of walking catfish sperm. Doi.org/10.54680/fr24510110612.

本刊更多论文

冷冻保护剂和浓度对冷冻保存后步行鲇精子质量的影响

背景：鲶鱼（Clarias batrachus）是印度尼西亚本土最受欢迎的淡水鲶鱼品种之一。然而，养殖面临着挑战，特别是由于养殖技术不发达导致幼体稀缺。低温保存是一种储存精子的方法，可以长期保持精子的活力，支持鱼类的繁殖技术。在这种情况下，冷冻保护剂在决定精子冷冻保存成功与否方面起着重要作用：材料与方法：共测试了五种不同类型的冷冻保护剂，即二甲基亚砜、甘油、乙二醇、乙醇和甲醇，浓度分别为 0%、5%、10%、15% 和 20%，每种冷冻保护剂有四个重复：低温保护剂的类型和浓度对精子活力和存活率有显著影响（P < 0.05）。5%二甲基亚砜和乙二醇、10%甘油和甲醇以及15%乙醇的效果最好：结论：5% 的二甲基亚砜可获得最高的活力和存活率，因此推荐用于冷冻保存步行鲇的精子。Doi.org/10.54680/fr24510110612.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.