Impact of different processing methods of Ligustrum lucidum Ait. on kidney-yin deficiency: a study based on pharmacodynamics and metabolomics research

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2024-08-10 DOI:10.1002/bmc.5969

Shu-ding Sun, Di Zhao, Xue-fang Liu, Wei-wei Zhang, Hao-ran Dong, Yan-ge Tian, Su-Xiang Feng

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Abstract

This study aimed to explore the pharmacodynamics and mechanisms of different processing methods of Ligustrum lucidum Ait. (LLA) in addressing kidney-yin deficiency (KYD). Forty-eight Sprague–Dawley rats were divided into eight groups based on their weight. The KYD model was established by intragastric administration of levothyroxine sodium. Each group was administered the corresponding treatment for 15 consecutive days. The general condition of the rats during the treatment period was observed. In addition, the levels of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), and the ratio of cAMP to cGMP in the serum of rats from different groups were measured. Serum samples were analyzed using the ultra-performance liquid chromatography (UPLC)-Orbitrap Fusion MS technique for metabolomics analysis. Compared with the model group, the general condition of the rats in the wine-steamed L. lucidum group (WL) and salt-steamed L. lucidum group (SSL) groups showed significant improvement. The serum levels of cAMP, cGMP, and the cAMP-to-cGMP ratio tended to return to normal. Metabolic analysis identified 38 relevant biomarkers and revealed 3 major metabolic pathways: phenylalanine, tyrosine, and tryptophan biosynthesis; phenylalanine metabolism; and sphingolipid metabolism. The different processing methods of LLA demonstrated therapeutic effects on KYD in rats, likely related to the restoration of disturbed metabolism by adjusting the levels of endogenous metabolites in the kidney. The SSL demonstrated significantly superior effects compared with the other four types of LLA processed products.

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女贞不同加工方法对肾阴虚的影响：基于药效学和代谢组学的研究

本研究旨在探讨女贞子（LLA）不同加工方法治疗肾阴虚（KYD）的药效学和机理。48只Sprague-Dawley大鼠按体重分为8组。通过胃内注射左甲状腺素钠建立肾阴虚模型。每组连续 15 天接受相应的治疗。观察大鼠在治疗期间的一般状况。此外，还测量了不同组大鼠血清中环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）的水平以及 cAMP 与 cGMP 的比值。血清样品采用超高效液相色谱（UPLC）- Orbitrap Fusion MS 技术进行代谢组学分析。与模型组相比，酒蒸组（WL）和盐蒸组（SSL）大鼠的一般状况有明显改善。血清中的cAMP、cGMP水平以及cAMP与cGMP的比值趋于恢复正常。代谢分析确定了 38 个相关生物标志物，并揭示了 3 个主要代谢途径：苯丙氨酸、酪氨酸和色氨酸的生物合成；苯丙氨酸代谢；鞘脂代谢。LLA 的不同加工方法对大鼠的 KYD 均有治疗效果，这可能与通过调整肾脏中的内源性代谢物水平来恢复紊乱的代谢有关。与其他四种 LLA 加工产品相比，SSL 的效果明显更胜一筹。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.