Enhancement of Docosahexaenoic Acid and Eicosapentaenoic Acid Biosynthesis in Isochrysis galbana by Bacillus jeotgali

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Marine Biotechnology Pub Date : 2024-08-09 DOI:10.1007/s10126-024-10337-5

Yijun Xu, Minnan Wu, Jiayi Cao, Yingying Wang, Lin Zhang, Xiaojun Yan, Yanrong Li, Jilin Xu

{"title":"Enhancement of Docosahexaenoic Acid and Eicosapentaenoic Acid Biosynthesis in Isochrysis galbana by Bacillus jeotgali","authors":"Yijun Xu, Minnan Wu, Jiayi Cao, Yingying Wang, Lin Zhang, Xiaojun Yan, Yanrong Li, Jilin Xu","doi":"10.1007/s10126-024-10337-5","DOIUrl":null,"url":null,"abstract":"<div>Isochrysis galbana is valuable in aquaculture due to its production of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). However, achieving high yields of polyunsaturated fatty acids (PUFAs) presents challenges, leading to exploration of innovative approaches. This study investigated the influence of Bacillus jeotgali on the growth of I. galbana and its fatty acid composition. Co-culturing I. galbana with B. jeotgali significantly increased chlorophyll a content and cell abundance, particularly at higher bacterial population densities (algae-to-bacteria ratio of 1:10). Physiological and biochemical analyses found elevated soluble protein content in microalgae co-cultured with B. jeotgali, accompanied by decreased superoxide dismutase (SOD) activity. Fatty acid composition analysis demonstrated a distinctive profile in co-cultured I. galbana, characterized by increased PUFAs, especially EPA and DHA. Gene expression analysis indicated an upregulation of desaturase genes (d4FAD, d5FAD, d6FAD, and d8FAD) associated with PUFA synthesis pathway in I. galbana during co-culturing with B. jeotgali. This study advances our understanding of bacteria-microalgae interactions and presents a promising strategy for enhancing the production of DHA and EPA.</div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 5","pages":"991 - 999"},"PeriodicalIF":2.6000,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-024-10337-5.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Biotechnology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10126-024-10337-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Isochrysis galbana is valuable in aquaculture due to its production of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). However, achieving high yields of polyunsaturated fatty acids (PUFAs) presents challenges, leading to exploration of innovative approaches. This study investigated the influence of Bacillus jeotgali on the growth of I. galbana and its fatty acid composition. Co-culturing I. galbana with B. jeotgali significantly increased chlorophyll a content and cell abundance, particularly at higher bacterial population densities (algae-to-bacteria ratio of 1:10). Physiological and biochemical analyses found elevated soluble protein content in microalgae co-cultured with B. jeotgali, accompanied by decreased superoxide dismutase (SOD) activity. Fatty acid composition analysis demonstrated a distinctive profile in co-cultured I. galbana, characterized by increased PUFAs, especially EPA and DHA. Gene expression analysis indicated an upregulation of desaturase genes (d4FAD, d5FAD, d6FAD, and d8FAD) associated with PUFA synthesis pathway in I. galbana during co-culturing with B. jeotgali. This study advances our understanding of bacteria-microalgae interactions and presents a promising strategy for enhancing the production of DHA and EPA.

Abstract Image

查看原文本刊更多论文

jeotgali 芽孢杆菌促进了半枝莲二十二碳六烯酸和二十碳五烯酸的合成。

伽蓝异核藻（Isochrysis galbana）可产生二十碳五烯酸（EPA）和二十二碳六烯酸（DHA），因此在水产养殖中很有价值。然而，实现多不饱和脂肪酸（PUFAs）的高产量是一项挑战，因此需要探索创新方法。本研究调查了枯草芽孢杆菌（Bacillus jeotgali）对加尔巴那藻类（I. galbana）生长及其脂肪酸组成的影响。将蚬贝与空肠芽孢杆菌共培养可显著提高叶绿素 a 含量和细胞丰度，尤其是在细菌种群密度较高的情况下（藻类与细菌的比例为 1:10）。生理和生化分析发现，与焦藻类共培养的微藻可溶性蛋白质含量增加，同时超氧化物歧化酶（SOD）活性降低。脂肪酸组成分析表明，与 I. galbana 共培养的微藻具有独特的特征，即增加了 PUFAs，尤其是 EPA 和 DHA。基因表达分析表明，在 I. galbana 与 B. jeotgali 的共培养过程中，与 PUFA 合成途径相关的去饱和酶基因（d4FAD、d5FAD、d6FAD 和 d8FAD）上调。这项研究加深了我们对细菌与微藻之间相互作用的了解，并为提高 DHA 和 EPA 的产量提供了一种前景广阔的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Marine Biotechnology 工程技术-海洋与淡水生物学

CiteScore

4.80

自引率

3.30%

发文量

审稿时长

2 months

期刊介绍： Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.