Tea-derived exosome-like nanoparticles prevent irritable bowel syndrome induced by water avoidance stress in rat model.

IF 3.7 3区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY

Journal of Gastroenterology and Hepatology Pub Date : 2024-08-09 DOI:10.1111/jgh.16714

Qianyuan Gong, Feng Xiong, Yaxian Zheng, Yuanbiao Guo

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引用次数: 0

Abstract

Background and aim: Exosome-like nanoparticles (ELNs) have emerged as crucial mediators of intercellular communication, evaluated as potential bioactive nutraceutical biomolecules. We hypothesized that oral ELNs have some therapeutic effect on irritable bowel syndrome (IBS).

Methods: In our study, ELNs from tea (Camellia sinensis) leaves were extracted by differential centrifugation. We investigated the role of ELNs by assessing visceral hypersensitivity, body weight, bowel habits, tight junctions, and corticotropin-releasing hormone (CRH) in rats subjected to water avoidance stress (WAS) to mimic IBS with and without ELNs (1 mg/kg per day) for 10 days.

Results: The average diameter of ELNs from LCC, FD and MZ tea tree were 165 ± 107, 168 ± 94, and 168 ± 108 nm, the concentration of ELNs were 1.2 × 10¹³, 1 × 10¹³, and 1.5 × 10¹³ particles/mL, respectively. ELNs can be taken up by intestinal epithelial cells. In WAS rats, ELNs significantly restored weight, recovered tight junctions, decreased CRH, and CRH receptor 1 expression levels and inhibited abdominal hypersensitivity in comparison to positive control.

Conclusions: Oral tea-derived ELN improves symptoms of IBS by potentially modulating the CRH pathway.

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茶源性外泌体纳米颗粒可预防大鼠模型因避水应激引起的肠易激综合征

背景和目的：外泌体类纳米颗粒（ELNs）已成为细胞间交流的重要媒介，被评估为潜在的生物活性营养生物大分子。我们假设口服ELNs对肠易激综合征（IBS）有一定的治疗作用：我们的研究采用差速离心法从茶叶中提取了ELNs。我们通过评估内脏超敏性、体重、排便习惯、紧密连接和促肾上腺皮质激素释放激素（CRH）来研究ELNs的作用：LCC、FD和MZ茶树ELNs的平均直径分别为165±107、168±94和168±108 nm，ELNs的浓度分别为1.2×1013、1×1013和1.5×1013微粒/毫升。ELNs 可被肠上皮细胞吸收。与阳性对照组相比，ELNs在WAS大鼠体内可显著恢复体重，恢复紧密连接，降低CRH和CRH受体1的表达水平，并抑制腹部超敏反应：结论：口服茶叶提取的ELN可通过调节CRH途径改善肠易激综合征的症状。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Gastroenterology and Hepatology 医学-胃肠肝病学

CiteScore

7.90

自引率

2.40%

发文量

326

审稿时长

2.3 months

期刊介绍： Journal of Gastroenterology and Hepatology is produced 12 times per year and publishes peer-reviewed original papers, reviews and editorials concerned with clinical practice and research in the fields of hepatology, gastroenterology and endoscopy. Papers cover the medical, radiological, pathological, biochemical, physiological and historical aspects of the subject areas. All submitted papers are reviewed by at least two referees expert in the field of the submitted paper.