Evaluation of Safe Margins Using Immunohistochemistry in Oral Squamous Cell Carcinoma.

IF 0.8 Q4 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Maxillofacial & Oral Surgery Pub Date : 2024-08-01 Epub Date: 2024-06-25 DOI:10.1007/s12663-024-02236-y

Aaisha Siddiqa, Divya Jivrajani, Neelakamal H Hallur, Shereen Fatima, Chaitanya Kothari, Sadanand Shivanna Kuntaraddi

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引用次数: 0

Abstract

Background: Hematoxylin & Eosin (H & E) stains have been conventionally used to establish the status of safe margins following resection of primary Oral Squamous Cell Carcinoma. Due to non-specificity of this stain, there is a possibility of false negative results. In this study, we have assessed the role of Immunohistochemistry (IHC) in establishing the status of safe margins.

Aim: To compare Hematoxylin & Eosin (H & E) and Immunohistochemistry (IHC) staining in identification of tumor cells in establishing the status of safe margins.

Methodology: This study included 14 cases diagnosed with OSCC. Following resection, the primary lesion was subjected to Histopathological analysis. 2 sets of HP slides were prepared from serial sectioning of the wax block prepared for each of the four margins. Both sets of slides were stained with H &E stain. One set of these slides was further stained with Pan CK marker (IHC) which is a cytokeratin marker to identify tumour cells.

Results: All the slides with H & E staining reported negative for tumor infiltration and 4 slides (3 patients) out of 56 were reported positive with PanCK marker. There was a statistically significant difference in the number of patients with positive margins using IHC as compared to H & E stain.

Conclusion: Immunohistochemistry using PanCK marker proved to be more efficient in the determination of status of safe margins than routine H & E staining.

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利用免疫组化评估口腔鳞状细胞癌的安全边缘

背景：血色素和伊红（H&E）染色一直被用于确定原发性口腔鳞状细胞癌切除术后的安全边缘状态。由于该染色法无特异性，因此可能出现假阴性结果。本研究评估了免疫组化（IHC）在确定安全边缘状态中的作用。目的：比较血红素和伊红（H&E）染色与免疫组化（IHC）染色在确定安全边缘状态中识别肿瘤细胞的作用：本研究包括14例确诊为OSCC的病例。切除后，对原发病灶进行组织病理学分析。对四个边缘的蜡块进行连续切片，分别制备两套 HP 切片。两组切片均采用 H & E 染色法染色。其中一组切片进一步用 Pan CK 标记（IHC）染色，这是一种细胞角蛋白标记，用于识别肿瘤细胞：结果：所有进行 H & E 染色的切片均显示肿瘤浸润为阴性，56 张切片中有 4 张（3 名患者）显示 PanCK 标记为阳性。与 H & E 染色相比，采用 IHC 检测的边缘阳性患者数量在统计学上有显著差异：结论：事实证明，使用 PanCK 标记的免疫组化在确定安全边缘的状态方面比常规 H & E 染色更有效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Maxillofacial & Oral Surgery DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

1.90

自引率

0.00%

发文量

138

期刊介绍： This journal offers comprehensive coverage of new techniques, important developments and innovative ideas in Oral and Maxillofacial Surgery. Practice-applicable articles help develop the methods used to handle dentoalveolar surgery, facial injuries and deformities, TMJ disorders, oral cancer, jaw reconstruction, anesthesia and analgesia. The journal also includes specifics on new instruments, diagnostic equipment’s and modern therapeutic drugs and devices. Journal of Oral and Maxillofacial Surgery is recommended for first or priority subscription by the Dental Section of the Medical Library Association. Specific topics covered recently have included: ? distraction osteogenesis ? synthetic bone substitutes ? fibroblast growth factors ? fetal wound healing ? skull base surgery ? computer-assisted surgery ? vascularized bone grafts Benefits to authorsWe also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services.