Unraveling immune cell heterogeneity in autoimmune arthritis: insights from single-cell RNA sequencing.

IF 2.7 Q3 IMMUNOLOGY
Immunological Medicine Pub Date : 2024-12-01 Epub Date: 2024-08-09 DOI:10.1080/25785826.2024.2388343
Sotaro Nakajima, Haruka Tsuchiya, Keishi Fujio
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引用次数: 0

Abstract

Single-cell RNA sequencing (scRNA-seq) has transformed our understanding of immune-mediated arthritis, which comprises rheumatoid arthritis and spondyloarthritis. This review outlines the key findings and advancements in scRNA-seq studies focused on the pathogenesis of autoimmune arthritis and its clinical application. In rheumatoid arthritis, scRNA-seq has elucidated the heterogeneity among synovial fibroblasts and immune cell subsets in inflammatory sites, offering insights into disease mechanisms and the differences in treatment responses. Various studies have identified distinct synovial fibroblast subpopulations, such as THY1+ inflammatory and THY1- destructive fibroblasts. Furthermore, scRNA-seq has revealed diverse T cell profiles in the synovium, including peripheral helper T cells and clonally expanded CD8+ T cells, shedding light on potential therapeutic targets and predictive markers of treatment response. Similarly, in spondyloarthritis, particularly psoriatic arthritis and ankylosing spondylitis, scRNA-seq studies have identified distinct cellular profiles associated with disease pathology. Challenges such as cost and sample size limitations persist, but collaborative efforts and utilization of public databases hold promise for overcoming these obstacles. Overall, scRNA-seq emerges as a powerful tool for dissecting cellular heterogeneity and driving precision medicine in immune-mediated arthritis.

揭示自身免疫性关节炎中免疫细胞的异质性:单细胞 RNA 测序的启示。
单细胞 RNA 测序(scRNA-seq)改变了我们对免疫介导的关节炎(包括类风湿性关节炎和脊柱关节炎)的认识。本综述概述了以自身免疫性关节炎发病机制及其临床应用为重点的 scRNA-seq 研究的主要发现和进展。在类风湿性关节炎中,scRNA-seq 阐明了炎症部位滑膜成纤维细胞和免疫细胞亚群之间的异质性,为疾病机制和治疗反应差异提供了见解。多项研究发现了不同的滑膜成纤维细胞亚群,如THY1+炎性成纤维细胞和THY1-破坏性成纤维细胞。此外,scRNA-seq 还揭示了滑膜中不同的 T 细胞特征,包括外周辅助性 T 细胞和克隆扩增的 CD8+ T 细胞,从而揭示了潜在的治疗靶点和治疗反应的预测标志物。同样,在脊柱关节炎,尤其是银屑病关节炎和强直性脊柱炎中,scRNA-seq 研究发现了与疾病病理相关的独特细胞特征。成本和样本量限制等挑战依然存在,但合作努力和利用公共数据库有望克服这些障碍。总之,scRNA-seq 是剖析细胞异质性和推动免疫介导的关节炎精准医疗的有力工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Immunological Medicine
Immunological Medicine Medicine-Immunology and Allergy
CiteScore
7.10
自引率
2.30%
发文量
19
审稿时长
19 weeks
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