Protective effect of Cordycepin on blood-testis barrier against pre-puberty polystyrene nanoplastics exposure in male rats.

IF 7.2 1区医学 Q1 TOXICOLOGY

Particle and Fibre Toxicology Pub Date : 2024-08-09 DOI:10.1186/s12989-024-00590-w

Ying Hu, Shuyi Jiang, Qiang Zhang, Wenjie Zhou, Jinhong Liang, Ying Xu, Wenhui Su

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Abstract

Plastic pollution is an emerging environmental issue, with microplastics and nanoplastics raising health concerns due to bioaccumulation. This work explored the impact of polystyrene nanoparticle (PS-NPs) exposure during prepuberty on male reproductive function post maturation in rats. Rats were gavaged with PS-NPs (80 nm) at 0, 3, 6, 12 mg/kg/day from postnatal day 21 to 95. PS-NPs accumulated in the testes and reduced sperm quality, serum reproductive hormones, and testicular coefficients. HE staining showed impaired spermatogenesis. PS-NPs disrupted the blood-testis barrier (BTB) by decreasing junction proteins, inducing inflammation and apoptosis. Transcriptomics identified differentially expressed genes related to metabolism, lysosome, apoptosis, and TLR4 signaling. Molecular docking revealed Cordycepin could compete with polystyrene for binding to TLR4. Cordycepin alleviated oxidative stress and improved barrier function in PS-NPs treated Sertoli cells. In conclusion, prepubertal PS-NPs exposure induces long-term reproductive toxicity in male rats, likely by disrupting spermatogenesis through oxidative stress and BTB damage. Cordycepin could potentially antagonize this effect by targeting TLR4 and warrants further study as a protective agent. This study elucidates the mechanisms underlying reproductive toxicity of PS-NPs and explores therapeutic strategies.

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虫草素对雄性大鼠青春期前接触聚苯乙烯纳米塑料对血液-睾丸屏障的保护作用

塑料污染是一个新出现的环境问题，微塑料和纳米塑料的生物蓄积性引起了人们对健康的关注。这项研究探讨了大鼠在青春期前接触聚苯乙烯纳米粒子（PS-NPs）对成熟后雄性生殖功能的影响。从大鼠出生后第 21 天到第 95 天，以 0、3、6、12 毫克/千克/天的剂量对其灌胃 PS-NPs（80 纳米）。PS-NPs 在睾丸中积累，降低了精子质量、血清生殖激素和睾丸系数。HE 染色显示精子发生受损。PS-NPs 通过减少连接蛋白、诱导炎症和细胞凋亡破坏了血睾屏障（BTB）。转录组学发现了与新陈代谢、溶酶体、细胞凋亡和 TLR4 信号转导有关的差异表达基因。分子对接显示 Cordycepin 可与聚苯乙烯竞争与 TLR4 的结合。Cordycepin 可减轻氧化应激并改善 PS-NPs 处理的 Sertoli 细胞的屏障功能。总之，青春期前接触 PS-NPs 可能会通过氧化应激和 BTB 损伤破坏精子发生，从而诱发雄性大鼠的长期生殖毒性。虫草素可能会通过靶向 TLR4 来拮抗这种效应，作为一种保护剂值得进一步研究。本研究阐明了 PS-NPs 生殖毒性的基本机制，并探讨了治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Particle and Fibre Toxicology TOXICOLOGY-

CiteScore

15.90

自引率

4.00%

发文量

审稿时长

6 months

期刊介绍： Particle and Fibre Toxicology is an online journal that is open access and peer-reviewed. It covers a range of disciplines such as material science, biomaterials, and nanomedicine, focusing on the toxicological effects of particles and fibres. The journal serves as a platform for scientific debate and communication among toxicologists and scientists from different fields who work with particle and fibre materials. The main objective of the journal is to deepen our understanding of the physico-chemical properties of particles, their potential for human exposure, and the resulting biological effects. It also addresses regulatory issues related to particle exposure in workplaces and the general environment. Moreover, the journal recognizes that there are various situations where particles can pose a toxicological threat, such as the use of old materials in new applications or the introduction of new materials altogether. By encompassing all these disciplines, Particle and Fibre Toxicology provides a comprehensive source for research in this field.