Unveiling thrips morphology: A comparative analysis of microscopy-based ultrastructural, morphological, and molecular characterization of Thrips tabaci and Thrips parvispinus in onion.

IF 2 3区工程技术 Q2 ANATOMY & MORPHOLOGY

Microscopy Research and Technique Pub Date : 2024-08-08 DOI:10.1002/jemt.24653

Arunkumar Pandi, Kannan Malaichamy, Kennedy John Samuel, Dineshkumar Selvaraj, Govindaraju Kasivelu, Elango Kolanthasamy

{"title":"Unveiling thrips morphology: A comparative analysis of microscopy-based ultrastructural, morphological, and molecular characterization of Thrips tabaci and Thrips parvispinus in onion.","authors":"Arunkumar Pandi, Kannan Malaichamy, Kennedy John Samuel, Dineshkumar Selvaraj, Govindaraju Kasivelu, Elango Kolanthasamy","doi":"10.1002/jemt.24653","DOIUrl":null,"url":null,"abstract":"The present study unveils the intricate details on the morphology of thrips through optical, field emission scanning electron microscopy (FE-SEM) and mitochondrial cytochrome oxidase I gene-based molecular identification tools. The variation in the morphological characters namely, antennae (seven-segmented with forked sensorium on third, fourth segments), ctenidia (paired ctenidia were present in 5th-8th abdominal segments laterally), pronotum (two pairs of posteroangular setae) were observed in both Thrips tabaci and Thrips parvispinus, respectively. Similarly, ocelli color (brown and red colored), ocellar setae (two and three pairs of ocellar setae on the head of T. tabaci and T. parvispinus, respectively. Irregular reticulate striations on metascutum and medial striations are present in the metanotum of T. parvispinus; forewings with 6 distal setae in the first vein and 15 distal setae in the second vein in T. tabaci and forewings of T. parvispinus with complete rows of setae in the first and second vein in T. parvispinus; abdomen with median dorsal setae present in the tergite of T. tabaci and presence of 6-12 discal setae in sternites III-VI segments, absence of discal setae on sternites II and VII in T. parvispinus were observed, respectively. Further, FE-SEM studies revealed that similar type of sensilla namely, sensilla basiconica (SBI, SBII, SBIII), sensilla chaetica (SChI, SChII), sensilla trichodea (ST), sensilla campaniformia (SCa), and sensilla cavity (SCav) were recorded in both the species and variations were observed in length of above sensilla of T. tabaci and T. parvispinus. Additionally, Bohm bristles (Bb) and microtrichia (Mt) on the antennal surface contributed to a comprehensive understanding of their ultrastructural features. The molecular characterization revealed a single ~450 bp nucleotide fragment with over 98% similarity for the confirmation of T. tabaci and T. parvispinus in concurrence with NCBI data. RESEARCH HIGHLIGHTS: Microscopy-based morphological and ultrastructural characterization of Thrips tabaci Lindeman and Thrips parvispinus Karny.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopy Research and Technique","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/jemt.24653","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The present study unveils the intricate details on the morphology of thrips through optical, field emission scanning electron microscopy (FE-SEM) and mitochondrial cytochrome oxidase I gene-based molecular identification tools. The variation in the morphological characters namely, antennae (seven-segmented with forked sensorium on third, fourth segments), ctenidia (paired ctenidia were present in 5^th-8^th abdominal segments laterally), pronotum (two pairs of posteroangular setae) were observed in both Thrips tabaci and Thrips parvispinus, respectively. Similarly, ocelli color (brown and red colored), ocellar setae (two and three pairs of ocellar setae on the head of T. tabaci and T. parvispinus, respectively. Irregular reticulate striations on metascutum and medial striations are present in the metanotum of T. parvispinus; forewings with 6 distal setae in the first vein and 15 distal setae in the second vein in T. tabaci and forewings of T. parvispinus with complete rows of setae in the first and second vein in T. parvispinus; abdomen with median dorsal setae present in the tergite of T. tabaci and presence of 6-12 discal setae in sternites III-VI segments, absence of discal setae on sternites II and VII in T. parvispinus were observed, respectively. Further, FE-SEM studies revealed that similar type of sensilla namely, sensilla basiconica (SBI, SBII, SBIII), sensilla chaetica (SChI, SChII), sensilla trichodea (ST), sensilla campaniformia (SCa), and sensilla cavity (SCav) were recorded in both the species and variations were observed in length of above sensilla of T. tabaci and T. parvispinus. Additionally, Bohm bristles (Bb) and microtrichia (Mt) on the antennal surface contributed to a comprehensive understanding of their ultrastructural features. The molecular characterization revealed a single ~450 bp nucleotide fragment with over 98% similarity for the confirmation of T. tabaci and T. parvispinus in concurrence with NCBI data. RESEARCH HIGHLIGHTS: Microscopy-based morphological and ultrastructural characterization of Thrips tabaci Lindeman and Thrips parvispinus Karny.

查看原文本刊更多论文

揭开蓟马形态的面纱：基于显微镜的洋葱蓟马和副蓟马超微结构、形态和分子特征比较分析。

本研究通过光学、场发射扫描电子显微镜（FE-SEM）和基于线粒体细胞色素氧化酶 I 基因的分子鉴定工具，揭示了蓟马形态的复杂细节。观察到烟青蓟马和副蓟马的形态特征存在差异，即触角（七节，第三、四节上有分叉的感应器）、栉体（成对的栉体出现在腹部第 5-8 节的侧面）、前胸（两对后角刚毛）。同样，T. tabaci 和 T. parvispinus 的眼轮颜色（棕色和红色）、眼轮刚毛（T. tabaci 和 T. parvispinus 头部分别有两对和三对眼轮刚毛。T. tabaci 的前翅第一脉有 6 根远端刚毛，第二脉有 15 根远端刚毛，T. parvispinus 的前翅第一脉和第二脉有完整的刚毛列。T.tabaci和 T. parvispinus的前翅第一和第二脉有完整的刚毛列；观察到 T. tabaci 的腹部有中背刚毛，胸片 III-VI 节有 6-12 根盘状刚毛，T. parvispinus 的胸片 II 和 VII 没有盘状刚毛。此外，FE-SEM 研究还发现，两个物种都有类似类型的感觉器，即基本感觉器（SBI、SBII、SBIII）、楔形感觉器（SChI、SChII）、毛状感觉器（ST）、钟状感觉器（SCa）和穴状感觉器（SCav），并观察到 T. tabaci 和 T. parvispinus 上述感觉器的长度存在差异。此外，触角表面的波姆刚毛（Bb）和微管（Mt）也有助于全面了解它们的超微结构特征。分子特征研究发现了一个 ~450 bp 的核苷酸片段，其相似度超过 98%，可用于确认 T. tabaci 和 T. parvispinus，与 NCBI 数据一致。研究亮点：基于显微镜的烟青蓟马（Thrips tabaci Lindeman）和副蓟马（Thrips parvispinus Karny）的形态学和超微结构特征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microscopy Research and Technique 医学-解剖学与形态学

CiteScore

5.30

自引率

20.00%

发文量

233

审稿时长

4.7 months

期刊介绍： Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.