Evaluation of gradient diffusion in vitro susceptibility testing of Aerococcus urinae

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
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引用次数: 0

Abstract

Objective

Aerococcus urinae antimicrobial susceptibility testing can be performed via broth microdilution with Mueller-Hinton broth supplemented with lysed horse blood. We sought to compare this with the commonly used gradient diffusion method.

Methods

We compared broth microdilution with Mueller-Hinton broth supplemented with lysed horse blood and gradient diffusion via Mueller-Hinton agar supplemented with sheep blood for 190 A. urinae isolates against 16 antimicrobials.

Results

No antimicrobials demonstrated more than 90% essential and categorical agreement, and fewer than 3% demonstrated major and very major error rates. Trimethoprim-sulfamethoxazole demonstrated an 81% major error rate and ceftriaxone demonstrated a 76% very major error rate. Agar dilution with lysed horse blood was performed for trimethoprim-sulfamethoxazole against 94 isolates and showed 100% susceptibility, consistent with previous studies.

Conclusions

Given its limitations in detecting resistant strains, our findings cannot support the routine use of gradient diffusion with Mueller-Hinton agar supplemented with sheep blood for A. urinae in lieu of the Clinical and Laboratory Standards Institute method. Our results suggest that A. urinae is usually susceptible to penicillin, linezolid, tetracycline, and vancomycin. Future studies should evaluate alternative testing methods for clinical microbiology laboratories.

对尿道球菌体外药敏试验梯度扩散的评估
目的:尿道气球菌抗菌药物敏感性测试(AST)可通过肉汤微稀释法(BMD)进行,使用的肉汤为添加了裂解马血的穆勒-欣顿肉汤(MHB-LHB)。我们试图将其与常用的梯度扩散法进行比较:方法:我们比较了使用 MHB-LHB 的 BMD 法和通过补充了绵羊血的 MH 琼脂进行梯度扩散的方法,对 190 株尿囊菌分离物进行了 16 种抗菌药物的检测:结果:没有一种抗菌药物的基本一致率和分类一致率(EA 和 CA)超过 90%,重大和非常重大错误率(ME 和 VME)低于 3%。甲氧苄啶-磺胺甲噁唑(TMP-STX)的重大错误率为 81%,头孢曲松的极重大错误率为 76%。用溶解马血进行琼脂稀释 (AD),对 94 个分离菌株进行 TMP-STX 检测,发现其药敏率为 100%,与之前的研究结果一致:我们的研究结果并不支持常规使用添加了绵羊血的 MH 琼脂梯度扩散法检测尿囊绦虫,以取代 CLSI 方法,因为该方法在检测耐药菌株方面存在局限性。我们的研究结果表明,A. urinae 通常对青霉素、利奈唑胺、四环素和万古霉素敏感。未来的研究应评估临床微生物实验室的其他检测方法。
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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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