Carbapenemase-producing Klebsiella quasipneumoniae ST688 (NDM-1) and Klebsiella michiganensis ST40 (KPC-2) in food destined for hospitalized patients

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
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Abstract

Objectives

Klebsiella spp. are leading causes of nosocomial infections. Their ability to harbour antimicrobial resistance genes makes them an important public health threat. This study aimed to report the genomic background of carbapenemase-producing Klebsiella quasipneumoniae (HV55B) and Klebsiella michiganensis (HV55D) strains isolated from fresh vegetables destined for hospitalized inpatients.

Methods

Microbiological and molecular methods were used to isolate and identify the strains, which were submitted to the antimicrobial susceptibility test and pH tolerance assays. Whole genome sequencing was performed on MiSeq and NextSeq platforms, and online available tools were applied to bioinformatic analysis of clinically relevant information.

Results

Both isolates were considered multidrug-resistant and tolerated pH ≥ 4 for 24 h. HV55B belonged to sequence type (ST) ST668, and presented a broad resistome and plasmids from four incompatibility groups. HV55D belonged to ST40. Both strains HV55B and HV55D were genetically close to isolates responsible for human infections around the world, which stands for the plausibility of such bacteria to cause disease in patients of the studied institution.

Conclusions

Our results confirm the presence of carbapenemase-producing Klebsiella spp. in fresh foodstuffs intended for hospitalized inpatients’ consumption. The genomes characterized here also provide clinically and genomically relevant information to forthcoming epidemiological surveillance studies.

供住院病人食用的食品中产碳青霉烯酶的类肺炎克雷伯菌 ST688(NDM-1)和密歇根克雷伯菌 ST40(KPC-2)。
目的:克雷伯氏菌属是引起医院内感染的主要原因。它们携带抗菌药耐药性基因的能力使其成为重要的公共卫生威胁。本研究旨在报告从供住院患者食用的新鲜蔬菜中分离出的产碳青霉烯酶的准肺炎克雷伯氏菌(HV55B)和密歇根克雷伯氏菌(HV55D)菌株的基因组背景:采用微生物学和分子方法分离和鉴定菌株,并对其进行抗菌药敏感性试验和 pH 耐受性试验。在 MiSeq 和 NextSeq 平台上进行了全基因组测序(WGS),并应用在线工具对临床相关信息进行了生物信息学分析:HV55B属于序列类型(ST)ST668,具有广泛的耐药基因组和来自四个不相容组的质粒。HV55D 属于 ST40。HV55B和HV55D两株菌株在基因上都与世界各地导致人类感染的分离菌株接近,这说明此类细菌可能会在研究机构的患者中致病:我们的研究结果证实,在供住院病人食用的新鲜食品中存在产碳青霉烯酶的克雷伯氏菌属。本文描述的基因组还为即将开展的流行病学监测研究提供了临床和基因组相关信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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