Isolation and characterization of novel acetogenic Moorella strains for employment as potential thermophilic biocatalysts.

IF 3.5 3区 生物学 Q2 MICROBIOLOGY
Tim Böer, Lisa Engelhardt, Alina Lüschen, Lena Eysell, Hiroki Yoshida, Dominik Schneider, Largus T Angenent, Mirko Basen, Rolf Daniel, Anja Poehlein
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引用次数: 0

Abstract

Thermophilic acetogenic bacteria have attracted attention as promising candidates for biotechnological applications such as syngas fermentation, microbial electrosynthesis, and methanol conversion. Here, we aimed to isolate and characterize novel thermophilic acetogens from diverse environments. Enrichment of heterotrophic and autotrophic acetogens was monitored by 16S rRNA gene-based bacterial community analysis. Seven novel Moorella strains were isolated and characterized by genomic and physiological analyses. Two Moorella humiferrea isolates showed considerable differences during autotrophic growth. The M. humiferrea LNE isolate (DSM 117358) fermented carbon monoxide (CO) to acetate, while the M. humiferrea OCP isolate (DSM 117359) transformed CO to hydrogen and carbon dioxide (H2 + CO2), employing the water-gas shift reaction. Another carboxydotrophic hydrogenogenic Moorella strain was isolated from the covering soil of an active charcoal burning pile and proposed as the type strain (ACPsT) of the novel species Moorella carbonis (DSM 116161T and CCOS 2103T). The remaining four novel strains were affiliated with Moorella thermoacetica and showed, together with the type strain DSM 2955T, the production of small amounts of ethanol from H2 + CO2 in addition to acetate. The physiological analyses of the novel Moorella strains revealed isolate-specific differences that considerably increase the knowledge base on thermophilic acetogens for future applications.

分离和鉴定新型醋酸菌株,将其用作潜在的嗜热生物催化剂。
嗜热醋酸菌作为合成气发酵、微生物电合成和甲醇转化等生物技术应用的有前途的候选菌种,已经引起了人们的关注。在此,我们旨在从不同环境中分离和鉴定新型嗜热醋酸菌。通过基于 16S rRNA 基因的细菌群落分析,监测了异养和自养醋酸菌的富集情况。通过基因组和生理学分析,分离并鉴定了七株新的 Moorella 菌株。其中两株Moorella humiferrea分离株在自养生长过程中表现出相当大的差异。M. humiferrea LNE 分离物(DSM 117358)将一氧化碳(CO)发酵成醋酸盐,而 M. humiferrea OCP 分离物(DSM 117359)则利用水气转移反应将 CO 转化成氢气和二氧化碳(H2 + CO2)。从活性炭燃烧堆的覆盖土壤中分离出了另一株羧营养产氢 Moorella 菌株,并将其作为新物种 Moorella carbonis 的模式菌株(ACPsT)(DSM 116161T,CCOS 2103T)。其余四株新菌株隶属于热乙酸莫雷拉菌,与模式菌株 DSM 2955T 一样,除了乙酸之外,还能从 H2 + CO2 中产生少量乙醇。对新型莫雷拉菌株的生理学分析表明,这些菌株的分离特异性差异大大增加了嗜热醋酸菌的知识库,有利于今后的应用。
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来源期刊
FEMS microbiology ecology
FEMS microbiology ecology 生物-微生物学
CiteScore
7.50
自引率
2.40%
发文量
132
审稿时长
3 months
期刊介绍: FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms
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