Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19: An observational multicenter cohort pilot study

IF 4.5 3区医学 Q2 IMMUNOLOGY

Clinical immunology Pub Date : 2024-08-06 DOI:10.1016/j.clim.2024.110336

Adin Sejdic , Hans Jakob Hartling , Jon Gitz Holler , Lars Klingen Gjærde , Arnold Matovu Dungu , Maria Elizabeth Engel Møller , Rebecca Svanberg Teglgaard , Carsten Utoft Utoft Niemann , Patrick Terrence Brooks , Trine H. Mogensen , Nina Weis , Daria Podlekareva , Marie Louise Baum Jørgensen , Anne Ortved Gang , Ditte Stampe Hersby , Annemette Hald , Susanne Dam Nielsen , Anne-Mette Lebech , Marie Helleberg , Jens Lundgren , Birgitte Lindegaard

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引用次数: 0

Abstract

Introduction

Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients.

Methods and results

This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of naïve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls.

Conclusions

Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.

查看原文本刊更多论文

COVID-19住院患者入院时按体重指数分层的深度免疫细胞表型和诱导免疫细胞反应：一项观察性多中心队列试点研究。

导言：超重和肥胖与 COVID-19 患者住院率和死亡率的增加有关。本研究旨在分析住院 COVID-19 患者的诱导免疫反应和深层免疫细胞特征，并按体重指数进行分层：这项观察性多中心队列试点研究纳入了丹麦 122 名经 PCR 证实患有 COVID-19 的成年患者，并按体重指数（正常体重、超重、肥胖）进行了分层。研究人员使用 TruCulture® 和流式细胞仪（使用定制的抗体面板 (DuraClone®)）对炎症和免疫细胞概况进行了评估。肥胖症患者的炎症表型更加趋向炎症，T细胞刺激后TNF-α、IL-8、IL-17和IL-10水平升高，B细胞图谱也发生了改变。与体重正常的患者和健康对照组相比，肥胖患者的幼稚型、过渡型、非同种型转换记忆B细胞和浆细胞的浓度更高：结论：COVID-19住院患者的肥胖可能与促炎细胞因子升高、抗炎IL-10和B细胞亚群活化增加有关，需要进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical immunology 医学-免疫学

CiteScore

12.30

自引率

1.20%

发文量

212

审稿时长

34 days

期刊介绍： Clinical Immunology publishes original research delving into the molecular and cellular foundations of immunological diseases. Additionally, the journal includes reviews covering timely subjects in basic immunology, along with case reports and letters to the editor.