Impact of hydrophobic modification on biocompatibility of Alaska pollock gelatin microparticles

IF 1.8 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences Pub Date : 2024-08-09 DOI:10.1007/s44211-024-00643-2

Ying Chuin Yee, Takeshi Mori, Shima Ito, Tetsushi Taguchi, Yoshiki Katayama

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Abstract

This study investigates the impact of hydrophobic modification on the immunogenicity, cytotoxicity, and inflammatory response of Alaska pollock gelatin (ApGltn) microparticles (MPs). Gelatin, known for its inherent biocompatibility, was modified with decyl group (C10) to explore potential alterations in its interaction with the immune system. Immunogenicity was evaluated through the measurement of material-specific IgM and IgG responses, indicating no significant increase post-modification. Cytotoxicity against Caco-2 cell lines and NF-κB-mediated LPS-induced inflammation were also assessed, revealing no exacerbation by the modified MPs. Furthermore, C10 modification with different types of linkage such as secondary amine and amide structure did not influence immune reactivity. These findings suggest that C10 modification maintains the non-immunogenicity and biocompatibility of gelatin MPs, supporting their potential use in biomedical applications.

Graphical abstract

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疏水改性对阿拉斯加狭鳕明胶微粒生物相容性的影响。

本研究探讨了疏水修饰对阿拉斯加狭鳕明胶（ApGltn）微颗粒（MPs）的免疫原性、细胞毒性和炎症反应的影响。明胶以其固有的生物相容性而闻名，用癸基（C10）对其进行改性，以探索其与免疫系统相互作用的潜在变化。通过测量材料特异性 IgM 和 IgG 反应来评估免疫原性，结果表明改性后的材料特异性 IgM 和 IgG 反应没有明显增加。还评估了 Caco-2 细胞系的细胞毒性和 NF-κB 介导的 LPS 诱导的炎症，结果显示改性 MPs 不会加剧炎症。此外，仲胺和酰胺结构等不同类型的 C10 连接修饰也不会影响免疫反应性。这些发现表明，C10 修饰保持了明胶多孔质材料的非免疫原性和生物相容性，支持其在生物医学应用中的潜在用途。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.