Multiplex antibiotic susceptibility testing of urinary tract infections using an electrochemical lab-on-a-chip

IF 3 4区医学 Q3 ENGINEERING, BIOMEDICAL

Biomedical Microdevices Pub Date : 2024-08-09 DOI:10.1007/s10544-024-00719-w

Benjamin Crane, Alex Iles, Craig E. Banks, Mamun Rashid, Patricia E. Linton, Kirsty J. Shaw

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Abstract

Urinary tract infections (UTIs) represent the most prevalent type of outpatient infection, with significant adverse health and economic burdens. Current culture-based antibiotic susceptibility testing can take up to 72 h resulting in ineffective prescription of broad-spectrum antibiotics, poor clinical outcomes and development of further antibiotic resistance. We report an electrochemical lab-on-a-chip (LOC) for testing samples against seven clinically-relevant antibiotics. The LOC contained eight chambers, each housing an antibiotic-loaded hydrogel (cephalexin, ceftriaxone, colistin, gentamicin, piperacillin, trimethoprim, vancomycin) or antibiotic-free control, alongside a resazurin bulk-modified screen-printed electrode for electrochemical detection of metabolically active bacteria using differential pulse voltammetry. Antibiotic susceptibility in simulated UTI samples or donated human urine with either Escherichia coli or Klebsiella pneumoniae could be established within 85 min. Incorporating electrochemical detection onto a LOC provides an inexpensive, simple method for the sensitive determination of antibiotic susceptibility that is significantly faster than using a culture-based approach.

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利用电化学芯片实验室对尿路感染进行多重抗生素药敏试验。

尿路感染（UTI）是最常见的门诊感染类型，对健康和经济造成严重的负面影响。目前以培养为基础的抗生素药敏测试可能需要 72 小时，结果导致广谱抗生素处方无效、临床疗效不佳以及抗生素耐药性的进一步发展。我们报告了一种电化学片上实验室（LOC），用于检测样本对七种临床相关抗生素的敏感性。该实验室芯片包含八个腔室，每个腔室都装有抗生素水凝胶（头孢氨苄、头孢曲松、可乐定、庆大霉素、哌拉西林、曲美普林、万古霉素）或不含抗生素的对照品，同时还有一个利马唑林改性丝网印刷电极，用于利用差分脉冲伏安法对代谢活跃的细菌进行电化学检测。可在 85 分钟内确定模拟 UTI 样品或捐赠人尿液中大肠埃希菌或肺炎克雷伯菌对抗生素的敏感性。在 LOC 中加入电化学检测技术可提供一种廉价、简单的方法，用于灵敏测定抗生素敏感性，其速度明显快于基于培养的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Microdevices 工程技术-工程：生物医学

CiteScore

6.90

自引率

3.60%

发文量

审稿时长

6 months

期刊介绍： Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology is an interdisciplinary periodical devoted to all aspects of research in the medical diagnostic and therapeutic applications of Micro-Electro-Mechanical Systems (BioMEMS) and nanotechnology for medicine and biology. General subjects of interest include the design, characterization, testing, modeling and clinical validation of microfabricated systems, and their integration on-chip and in larger functional units. The specific interests of the Journal include systems for neural stimulation and recording, bioseparation technologies such as nanofilters and electrophoretic equipment, miniaturized analytic and DNA identification systems, biosensors, and micro/nanotechnologies for cell and tissue research, tissue engineering, cell transplantation, and the controlled release of drugs and biological molecules. Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices and nanotechnology are encouraged. A non-exhaustive list of fields of interest includes: nanoparticle synthesis, characterization, and validation of therapeutic or imaging efficacy in animal models; biocompatibility; biochemical modification of microfabricated devices, with reference to non-specific protein adsorption, and the active immobilization and patterning of proteins on micro/nanofabricated surfaces; the dynamics of fluids in micro-and-nano-fabricated channels; the electromechanical and structural response of micro/nanofabricated systems; the interactions of microdevices with cells and tissues, including biocompatibility and biodegradation studies; variations in the characteristics of the systems as a function of the micro/nanofabrication parameters.