Probiotic and anti-inflammatory properties of Lactiplantibacillus plantarum MKTJ24 isolated from an artisanal fermented fish of North-east India

IF 4.5 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

New biotechnology Pub Date : 2024-08-06 DOI:10.1016/j.nbt.2024.07.005

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引用次数: 0

Abstract

The study aimed to isolate and characterize lactic acid bacteria from various traditional fermented fish products from North East India, including Xindol, Hentak, and Ngari, which hold significant dietary importance for the indigenous tribes. Additionally, the study sought to examine their untargeted metabolomic profiles. A total of 43 strains of Bacillus, Priestia, Staphylococcus, Pediococcus, and Lactiplantibacillus were isolated, characterized by 16 S rRNA gene and tested for probiotic properties. Five strains passed pH and bile salt tests with strain dependent antimicrobial activity, which exhibited moderate autoaggregation and hydrophobicity properties. Lactiplantibacillus plantarum MKTJ24 exhibited the highest hydrophobicity (42 %), which was further confirmed by adhesion assay in HT-29 cell lines (100 %). Lactiplantibacillus plantarum MKTJ24 treatment in LPS-stimulated HT-29 cells up-regulated expression of mucin genes compared to LPS-treated cells. Treatment of RAW 264.7 cells with Lactiplantibacillus plantarum MKTJ24 decreased LPS-induced reactive oxygen species (ROS) and nitric oxide (NO) productions. Further, genome analysis of Lactiplantibacillus plantarum MKTJ24 revealed the presence of several probiotic markers and immunomodulatory genes. The genome was found to harbor plantaricin operon involved in bacteriocin production. A pangenome analysis using all the publicly available L. plantarum genomes specifically isolated from fermented fish products identified 120 unique genes in Lactiplantibacillus plantarum MKTJ24. Metabolomic analysis indicated dominance of ascorbic acids, pentafluropropionate, cyclopropaneacetic acid, florobenzylamine, and furanone in Xindol. This study suggests that Lactiplantibacillus plantarum MKTJ24 has potential probiotic and immunomodulatory properties that could be used in processing traditional fermented fish products on an industrial scale to improve their quality and enhance functional properties.

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从印度东北部手工发酵鱼中分离出的植物乳杆菌 MKTJ24 的益生菌和抗炎特性。

该研究旨在从印度东北部的各种传统发酵鱼产品中分离乳酸菌并确定其特征，包括对土著部落具有重要饮食意义的 Xindol、Hentak 和 Ngari。此外，该研究还试图检测它们的非目标代谢组学特征。研究人员共分离出 43 株芽孢杆菌、普里斯特菌、葡萄球菌、木薯球菌和乳杆菌，通过 16S rRNA 基因对其进行鉴定，并测试其益生菌特性。五株菌株通过了 pH 和胆盐测试，具有菌株依赖性抗菌活性，表现出适度的自身聚集和疏水特性。植物乳杆菌 MKTJ24 的疏水性最高（42%），在 HT-29 细胞系中的粘附试验进一步证实了这一点（100%）。与 LPS 处理的细胞相比，植物乳杆菌 MKTJ24 处理 LPS 刺激的 HT-29 细胞可上调粘蛋白基因的表达。用植物乳杆菌 MKTJ24 处理 RAW 264.7 细胞可减少 LPS 诱导的活性氧（ROS）和一氧化氮（NO）的产生。此外，植物乳杆菌 MKTJ24 的基因组分析表明，它含有多种益生菌标记和免疫调节基因。基因组中还发现了参与细菌素生产的植物素操作子。利用所有公开的植物乳杆菌基因组（专门从发酵鱼类产品中分离出来）进行的泛基因组分析，在植物乳杆菌 MKTJ24 中发现了 120 个独特的基因。代谢组分析表明，抗坏血酸、五氟丙酸、环丙烷乙酸、氟苄胺和呋喃酮在鑫多中占主导地位。这项研究表明，植物乳杆菌 MKTJ24 具有潜在的益生菌和免疫调节特性，可用于工业规模的传统发酵鱼产品加工，以提高其质量和功能特性。

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来源期刊

New biotechnology 生物-生化研究方法

CiteScore

11.40

自引率

1.90%

发文量

审稿时长

1 months

期刊介绍： New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international. The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.