Bright New Resources for Syphilis Research: Genetically Encoded Fluorescent Tags for Treponema pallidum and Sf1Ep Cells.

IF 2.6 2区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Microbiology Pub Date : 2024-10-01 Epub Date: 2024-08-08 DOI:10.1111/mmi.15304

Linda Grillová, Emily Romeis, Nicole A P Lieberman, Lauren C Tantalo, Linda H Xu, Barbara Molini, Aldo T Trejos, George Lacey, David Goulding, Nicholas R Thomson, Alexander L Greninger, Lorenzo Giacani

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Abstract

The recently discovered methodologies to cultivate and genetically manipulate Treponema pallidum subsp. pallidum (T. pallidum) have significantly helped syphilis research, allowing the in vitro evaluation of antibiotic efficacy, performance of controlled studies to assess differential treponemal gene expression, and generation of loss-of-function mutants to evaluate the contribution of specific genetic loci to T. pallidum virulence. Building on this progress, we engineered the T. pallidum SS14 strain to express a red-shifted green fluorescent protein (GFP) and Sf1Ep cells to express mCherry and blue fluorescent protein (BFP) for enhanced visualization. These new resources improve microscopy- and cell sorting-based applications for T. pallidum, better capturing the physical interaction between the host and pathogen, among other possibilities. Continued efforts to develop and share new tools and resources are required to help our overall knowledge of T. pallidum biology and syphilis pathogenesis reach that of other bacterial pathogens, including spirochetes.

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梅毒研究的崭新资源：用于苍白螺旋体和 Sf1Ep 细胞的基因编码荧光标签

最近发现的培养和遗传操作苍白螺旋体亚种（T. pallidum）的方法极大地帮助了梅毒研究，使抗生素疗效的体外评估、评估不同苍白螺旋体基因表达的对照研究以及评估特定基因位点对苍白螺旋体毒力贡献的功能缺失突变体的产生成为可能。在这一进展的基础上，我们改造了 T. pallidum SS14 株系，使其表达红移绿色荧光蛋白（GFP），并改造了 Sf1Ep 细胞，使其表达 mCherry 和蓝色荧光蛋白（BFP），以增强可视性。这些新资源改进了基于显微镜和细胞分拣的苍白球病毒应用，更好地捕捉了宿主与病原体之间的物理相互作用以及其他可能性。我们需要继续努力开发和共享新的工具和资源，以帮助我们对苍白螺旋体生物学和梅毒致病机理的全面了解达到其他细菌病原体（包括螺旋体）的水平。

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来源期刊

Molecular Microbiology 生物-生化与分子生物学

CiteScore

7.20

自引率

5.60%

发文量

132

审稿时长

1.7 months

期刊介绍： Molecular Microbiology, the leading primary journal in the microbial sciences, publishes molecular studies of Bacteria, Archaea, eukaryotic microorganisms, and their viruses. Research papers should lead to a deeper understanding of the molecular principles underlying basic physiological processes or mechanisms. Appropriate topics include gene expression and regulation, pathogenicity and virulence, physiology and metabolism, synthesis of macromolecules (proteins, nucleic acids, lipids, polysaccharides, etc), cell biology and subcellular organization, membrane biogenesis and function, traffic and transport, cell-cell communication and signalling pathways, evolution and gene transfer. Articles focused on host responses (cellular or immunological) to pathogens or on microbial ecology should be directed to our sister journals Cellular Microbiology and Environmental Microbiology, respectively.