The Effect of Bone Marrow Mesenchymal Stem Cells on Nestin and Sox-2 Gene Expression and Spatial Learning (Percent Alternation Y-Maze Test) against AlCl3-Induced Alzheimer's-like Pathology in a Rat Model.

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL
Annita, Gusti Revilla, Hirowati Ali, Almurdi
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引用次数: 0

Abstract

Background: Alzheimer's disease (AD) is a neurodegenerative condition characterized by gradual cognitive impairment, including loss of synapses and nerve cells involved in learning, memory, and habit formation processes. Bone Marrow Mesenchymal Stem Cells (BM-MSCs) are multipotent cells. Because of their self-renewable, differentiation, and immunomodulatory capabilities, they are commonly used to treat many disorders. Hence, the current study intends to examine the effect of BM-MSCs transplantation on Aluminum chloride (AlCl3)-induced cognitive problems, an experimental model resembling AD's hallmarks in rats.

Methods: The study was conducted in 2022 at The Biomedical Laboratory Faculty of Medicine, Andalas University, Indonesia. Adult male Wistar rats (three groups: negative control; no intervention+treatment with PBS; positive control: AlCl3+treatment with aqua dest; AlCl3+BM-MSCs: AlCl3+treatment with BM-MSCs, n=5 each) were treated daily with AlCl3 orally for five days. Stem cells were intraperitoneally injected into rats at a dose of 1x106 cells/rat. The same quantity of phosphate-buffered saline was given to the control group. One month after stem cell injection, the rat brain tissue was removed and placed in the film bottles that had been created. The expression of neural progenitor cell markers, including nestin and sex-determining Y-box 2 (SOX-2), was analyzed using real-time polymerase chain reaction (RT-PCR). Rats' cognitive and functional memory were examined using Y-maze. Data were analyzed using SPSS software (version 26.0) with a one-way analysis of variance (ANOVA) test.

Results: The gene expression of nestin (29.74±0.42), SOX-2 (31.44±0.67), and percent alternation of Y-maze (67.04±2.28) increased in the AlCl3+BM-MSCs group compared to that in the positive control group. RT-PCR analysis indicated that nestin (P<0.001) and SOX-2 (P<0.001) were significantly enhanced in the AlCl3+BM-MSCs group compared to the positive control group. This group also indicated an increased percent alternation of Y-maze (P<0.001) in the AlCl3+BM-MSCs group compared to the positive control group.

Conclusion: Due to its potential effects on cell therapy, BM-MSCs were found effective in a rat model of AD on the impairment of the rats' behavior and increased expression of neural progenitor cell markers.

骨髓间充质干细胞对大鼠模型中 Nestin 和 Sox-2 基因表达以及空间学习能力(百分比交替 Y-迷宫试验)的影响,对抗 AlCl3 诱导的阿尔茨海默氏症样病理学。
背景:阿尔茨海默病(AD)是一种神经退行性疾病,其特征是认知功能逐渐受损,包括参与学习、记忆和习惯形成过程的突触和神经细胞丧失。骨髓间充质干细胞(BM-MSCs)是一种多能细胞。由于它们具有自我更新、分化和免疫调节能力,因此常用于治疗多种疾病。因此,本研究旨在探讨移植骨髓间充质干细胞对氯化铝(AlCl3)诱导的认知问题(一种类似于注意力缺失症特征的大鼠实验模型)的影响:研究于2022年在印度尼西亚安达卢西亚大学医学院生物医学实验室进行。成年雄性 Wistar 大鼠(三组:阴性对照组;无干预+PBS 处理组;阳性对照组:AlCl3+BM-间充质干细胞:每天口服 AlCl3,连续五天。大鼠腹腔注射干细胞,剂量为 1x106 个细胞/只。对照组给予相同剂量的磷酸盐缓冲盐水。注射干细胞一个月后,取出大鼠脑组织并将其放入已制作好的薄膜瓶中。使用实时聚合酶链反应(RT-PCR)分析神经祖细胞标记物的表达,包括nestin和性别决定Y-box 2(SOX-2)。使用Y-迷宫对大鼠的认知和功能记忆进行了检测。数据采用 SPSS 软件(26.0 版)进行分析,并进行单因素方差分析(ANOVA)检验:结果:与阳性对照组相比,AlCl3+BM-MSCs组的nestin(29.74±0.42)、SOX-2(31.44±0.67)和Y-迷宫交替率(67.04±2.28)的基因表达量均有所增加。RT-PCR分析表明,与阳性对照组相比,P3+BM-间充质干细胞组的nestin(P3+BM-间充质干细胞)增加了。该组还表明,与阳性对照组相比,P3+BM-MSCs 组的 Y-迷宫交替率有所增加:结论:BM-间充质干细胞在细胞治疗方面具有潜在的作用,它对AD大鼠模型中大鼠的行为障碍和神经祖细胞标志物表达的增加有效。
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来源期刊
Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
0.00%
发文量
84
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of com­muni­cation for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science re­search experiences on prevalent diseases in the region and analysis of various regional problems.
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