Association between Methylenetetrahydrofolate Reductase (MTHFR) and 5-Methyltetrahydrofolate-Homocysteine Methyltransferase Reductase (MTRR) Polymorphisms in Iraqi Patients with COVID-19.

IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL
Sura F Alsaffar, Noor Nihad Baqer
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引用次数: 0

Abstract

Background: The methylenetetrahydrofolate reductase (MTHFR) gene is an essential gene in the metabolism of folate-homocysteine. Recently, the level of homocysteine was found to be a significant marker in the follow-up of COVID-19 infection. Thus, this study aimed to detect the effect of genetic polymorphisms for single nucleotide polymorphisms (SNPs) (c.66A>G, c.1298A>C, and c.677CT) on COVID-19 infection.

Methods: Blood samples were collected from 270 patients with COVID-19 in the medical center of Al-Shifa (Baghdad, Iraq) from November 2020 to March 2021. Tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was used for the detection of genotypes of SNPs. The odds ratio (OR) was used to detect the relationship between SNPs and COVID-19 infections. Haplotype analysis was performed by SHEsis software.

Results: There was a significant difference between mild/moderate cases and severe/critical cases for ages (35-45), (46-55), and (56-65) years (P<0.0001, P=0.01, and P=0.006, respectively). The results showed significant differences in the T allele for SNP c.677>C (P<0.0001 and OR=4.58). The C allele for SNP c.1298A>C indicated significant differences (P<0.001 and OR=3.15). Besides, the G allele for SNP c.677C>T showed significant differences (P<0.001 and OR=6.64). Consequently, these SNPs showed a predisposition to the development of COVID-19 infection. With regard to the C-A-A, T-A-A and T-C-G haplotypes indicated significant differences between the control and patient groups. The C-A-A was related to a decreased risk and indicated a protective effect against COVID-19 infection development (P<0.0001 and OR=0.218). The increased risk was associated with T-A-A and T-C-G haplotypes and indicated the risk impact on COVID-19 infection development (P<0.0001, P=0.004, and OR=15.5, OR=6.772, respectively). Furthermore, the linkage disequilibrium (LD) for SNPs was studied, and the complete D' value was 99.

Conclusion: The genetic polymorphisms of SNPs (c.66A>G, c.1298A>C, and c.677C>T) in the Iraqi population were associated with COVID-19 infection.

伊拉克 COVID-19 患者的亚甲基四氢叶酸还原酶 (MTHFR) 和 5-甲基四氢叶酸-高半胱氨酸甲基转移酶还原酶 (MTRR) 多态性之间的关联。
背景:亚甲基四氢叶酸还原酶(MTHFR)基因是叶酸-同型半胱氨酸代谢过程中的重要基因。最近,研究发现同型半胱氨酸水平是 COVID-19 感染随访的一个重要标志。因此,本研究旨在检测单核苷酸多态性(SNPs)(c.66A>G、c.1298A>C 和 c.677CT)对 COVID-19 感染的影响:2020年11月至2021年3月期间,在Al-Shifa医疗中心(伊拉克巴格达)采集了270名COVID-19患者的血样。采用四引物扩增难治性突变系统聚合酶链反应(ARMS-PCR)技术检测 SNPs 的基因型。采用几率比(OR)检测 SNP 与 COVID-19 感染之间的关系。用SHEsis软件进行单倍型分析:结果:轻度/中度病例与重度/危重病例在年龄(35-45 岁)、(46-55 岁)和(56-65 岁)之间存在显著差异(PC 表明存在显著差异(PT 表明存在显著差异(PConclusion)):伊拉克人群中的 SNPs(c.66A>G、c.1298A>C 和 c.677C>T)基因多态性与 COVID-19 感染有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
3.20
自引率
0.00%
发文量
84
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of com­muni­cation for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science re­search experiences on prevalent diseases in the region and analysis of various regional problems.
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