A practical clinical device for evaluating changes to the skin's sebum from ozone

IF 2.7 4区医学 Q2 DERMATOLOGY

International Journal of Cosmetic Science Pub Date : 2024-08-07 DOI:10.1111/ics.12998

Frank Liebel, Stephanie Petersen, Gary Grove, Tim Houser

{"title":"A practical clinical device for evaluating changes to the skin's sebum from ozone","authors":"Frank Liebel, Stephanie Petersen, Gary Grove, Tim Houser","doi":"10.1111/ics.12998","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>It is known that tropospheric ozone (O<sub>3</sub>) generated from pollutants reacting with UV forms lipid peroxidation products and induces oxidative stress to the skin. With the ever-increasing consumer awareness of the effects pollution has on skin, more testing methods will be needed to evaluate cosmetic ingredients. Recently, others have shown how antioxidants are able to reduce the effects from ozone on skin through in vitro, ex vivo and clinical studies where human subjects place their arms into large stationary chambers.</p>\n </section>\n \n <section>\n \n <h3> Aims</h3>\n \n <p>To develop a small, easy to use ozone exposure module (OEM) that can be used on various sites of the body and to validate this device for use in testing the ability of topical products to mitigate the effects of ozone exposure on the skin.</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>We have produced an OEM which can generate levels of ozone in excess of 1000 ppb and can be set to achieve the equivalent exposure to what is found in polluted environmental conditions. After exposure we used D-squame discs to remove the sebum and analytically quantitate squalene depletion. Squalene, which is very sensitive to ROS, easily oxidizes into early metabolite squalene monohydroperoxide (SQOOH) with ozone exposure.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>We were able to show decreases in squalene levels after exposure and protective effects from a topical formulation.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>This generator will be a useful tool for researchers to easily create a small and safe exposure from ozone for clinical testing.</p>\n </section>\n </div>","PeriodicalId":13936,"journal":{"name":"International Journal of Cosmetic Science","volume":"46 4","pages":"598-602"},"PeriodicalIF":2.7000,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Cosmetic Science","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/ics.12998","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

It is known that tropospheric ozone (O₃) generated from pollutants reacting with UV forms lipid peroxidation products and induces oxidative stress to the skin. With the ever-increasing consumer awareness of the effects pollution has on skin, more testing methods will be needed to evaluate cosmetic ingredients. Recently, others have shown how antioxidants are able to reduce the effects from ozone on skin through in vitro, ex vivo and clinical studies where human subjects place their arms into large stationary chambers.

Aims

To develop a small, easy to use ozone exposure module (OEM) that can be used on various sites of the body and to validate this device for use in testing the ability of topical products to mitigate the effects of ozone exposure on the skin.

Materials and Methods

We have produced an OEM which can generate levels of ozone in excess of 1000 ppb and can be set to achieve the equivalent exposure to what is found in polluted environmental conditions. After exposure we used D-squame discs to remove the sebum and analytically quantitate squalene depletion. Squalene, which is very sensitive to ROS, easily oxidizes into early metabolite squalene monohydroperoxide (SQOOH) with ozone exposure.

Results

We were able to show decreases in squalene levels after exposure and protective effects from a topical formulation.

Conclusion

This generator will be a useful tool for researchers to easily create a small and safe exposure from ozone for clinical testing.

查看原文本刊更多论文

用于评估臭氧对皮肤皮脂造成的变化的实用临床设备。

背景：众所周知，污染物与紫外线反应产生的对流层臭氧（O3）会形成脂质过氧化产物，诱发皮肤氧化应激。随着消费者越来越意识到污染对皮肤的影响，需要更多的测试方法来评估化妆品成分。最近，其他研究人员通过体外、体内和临床研究，证明了抗氧化剂如何能够减轻臭氧对皮肤的影响。目的：开发一种小型、易于使用的臭氧暴露模块（OEM），可用于人体的不同部位，并验证该设备是否可用于测试外用产品减轻臭氧暴露对皮肤影响的能力：我们制作了一个 OEM，它可以产生超过 1000 ppb 的臭氧，并可以设置成与污染环境条件下的臭氧暴露相当。暴露后，我们使用 D-squame 盘去除皮脂，并对角鲨烯的消耗进行定量分析。角鲨烯对 ROS 非常敏感，在接触臭氧后很容易氧化成早期代谢物角鲨烯单过氧化氢（SQOOH）：结果：我们发现，暴露于臭氧后，角鲨烯水平会下降，而外用制剂则具有保护作用：该发生器将成为研究人员的有用工具，可轻松地为临床测试创造少量安全的臭氧暴露。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Cosmetic Science DERMATOLOGY-

CiteScore

4.60

自引率

4.30%

发文量

期刊介绍： The Journal publishes original refereed papers, review papers and correspondence in the fields of cosmetic research. It is read by practising cosmetic scientists and dermatologists, as well as specialists in more diverse disciplines that are developing new products which contact the skin, hair, nails or mucous membranes. The aim of the Journal is to present current scientific research, both pure and applied, in: cosmetics, toiletries, perfumery and allied fields. Areas that are of particular interest include: studies in skin physiology and interactions with cosmetic ingredients, innovation in claim substantiation methods (in silico, in vitro, ex vivo, in vivo), human and in vitro safety testing of cosmetic ingredients and products, physical chemistry and technology of emulsion and dispersed systems, theory and application of surfactants, new developments in olfactive research, aerosol technology and selected aspects of analytical chemistry.