The Rpfor gene modulates the locomotory activity and host-seeking behaviour of Rhodnius prolixus.

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Newmar P Marliére, Marcelo G Lorenzo, Alessandra A Guarneri
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引用次数: 0

Abstract

The molecular bases of animal behaviour are intricate due to the pleiotropic nature of behaviour-modulating genes, which are often expressed across multiple tissues. The foraging gene (for) encodes a cGMP-dependent protein kinase (PKG), pivotal in regulating downstream target proteins through phosphorylation. In insects, for has been implicated in various behavioural contexts and physiological processes regarding searching for food. Rhodnius prolixus, a hematophagous bug that transmits Trypanosoma cruzi, the causative agent of Chagas disease, exhibits specific activity patterns associated with its hematophagous behaviour. Our previous work demonstrated a correlation between locomotor activity profiles and the expression of Rpfor, suggesting its involvement in modulating triatomine locomotion. In this study, we investigated the impact of Rpfor knockdown on locomotory activity, host-seeking behaviour, feeding performance and lipid metabolism in R. prolixus nymphs. Using RNA interference, we achieved a significant reduction of Rpfor expression in both the brain and fat body of R. prolixus nymphs. Knocked-down nymphs exhibited diminished non-oriented locomotory activity compared with controls, without altering the characteristic bimodal pattern of activity. Additionally, they displayed an increased tendency to approach a host, suggesting a role for Rpfor in modulating host-seeking behaviour. Feeding performance and lipid metabolism remained unaffected by Rpfor knockdown. Our findings underscore the multifaceted role of Rpfor in modulating locomotor activity and host-seeking behaviour in R. prolixus nymphs, shedding light on the molecular mechanisms underlying their hematophagous behaviour and potential implications for disease transmission. Further research is necessary to elucidate the intricate interplay between Rpfor expression, behaviour and physiological processes in triatomine bugs.

Rpfor基因调节了Rhodnius prolixus的运动活性和寻找宿主的行为。
动物行为的分子基础错综复杂,这是因为行为调节基因具有多效性,通常在多个组织中表达。觅食基因(for)编码一种 cGMP 依赖性蛋白激酶(PKG),在通过磷酸化调节下游靶蛋白方面起着关键作用。在昆虫中,觅食基因与寻找食物的各种行为和生理过程有关。Rhodnius prolixus 是一种食血虫,传播南美锥虫病的病原体--克鲁斯 Trypanosoma cruzi,它表现出与食血行为相关的特定活动模式。我们之前的研究表明,运动活动特征与 Rpfor 的表达之间存在相关性,这表明 Rpfor 参与调节三蠹的运动。在本研究中,我们研究了 Rpfor 基因敲除对 R. prolixus 若虫的运动活动、寻找宿主行为、摄食表现和脂质代谢的影响。通过 RNA 干扰,我们显著降低了 Rpfor 在 R. prolixus 若虫脑部和脂肪体中的表达。与对照组相比,被敲除的若虫表现出较低的非定向运动活动,但没有改变其特有的双峰活动模式。此外,它们接近宿主的倾向增加,这表明 Rpfor 在调节宿主寻找行为方面发挥了作用。Rpfor基因敲除对摄食性能和脂质代谢没有影响。我们的发现强调了 Rpfor 在调节 R. prolixus 若虫的运动活动和寻找宿主行为中的多方面作用,揭示了其噬血行为的分子机制以及对疾病传播的潜在影响。有必要开展进一步研究,以阐明三蠹中 Rpfor 表达、行为和生理过程之间错综复杂的相互作用。
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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