Screening of novel disease genes of sepsis-induced myocardial Disfunction by RNA sequencing and bioinformatics analysis

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Hanyi Yao , Zixi Xiao , Shufang Liu , Xingjian Gao , Zehong Wu , Dongping Li , Zhangqing Yi , Haojie Zhou , Weizhi Zhang
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引用次数: 0

Abstract

Background

There is still a lack of effective treatment for sepsis-induced myocardial dysfunction (SIMD), while the pathogenesis of SIMD still remains largely unexplained.

Methods

RNA sequencing results (GSE267388 and GSE79962) were used for cross-species integrative analysis. Bioinformatic analyses were used to delve into function, tissue- and cell- specificity, and interactions of genes. External datasets and qRT-PCR experiments were used for validation. L1000 FWD was used to predict targeted drugs, and 3D structure files were used for molecular docking.

Results

Based on bioinformatic analyses, ten differentially expressed genes were selected as genes of interest, seven of which were verified to be significantly differential expression. Bucladesine was considered as a potential targeted drug for SIMD, which banded to seven target proteins primarily by forming hydrogen bonds.

Conclusion

It was considered that Cebpd, Timp1, Pnp, Osmr, Tgm2, Cp, and Asb2 were novel disease genes, while bucladesine was a potential therapeutic drug, of SIMD.

通过RNA测序和生物信息学分析筛选脓毒症诱发心肌功能障碍的新型疾病基因
背景:脓毒症诱发的心肌功能障碍(SIMD)仍缺乏有效的治疗方法,而SIMD的发病机制在很大程度上仍未解释:方法:利用 RNA 测序结果(GSE267388 和 GSE79962)进行跨物种整合分析。生物信息分析用于深入研究基因的功能、组织和细胞特异性以及相互作用。外部数据集和 qRT-PCR 实验用于验证。使用 L1000 FWD 预测靶向药物,并使用三维结构文件进行分子对接:结果:根据生物信息学分析,选择了 10 个差异表达基因作为相关基因,其中 7 个基因被证实为显著差异表达。Bucladesine被认为是SIMD的潜在靶向药物,它主要通过形成氢键与七个靶蛋白结合:结论:Cebpd、Timp1、Pnp、Osmr、Tgm2、Cp和Asb2被认为是新的疾病基因,而bucladesine则是治疗SIMD的潜在药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
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