Phytochemical profiling and antioxidant evaluation of Rhododendron arboreum Sm leaf and flower: integrative analysis using advanced analytical techniques.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-07-01 Epub Date: 2024-08-16 DOI:10.1080/03639045.2024.2390029

Yangchen Dolma Kom, Karthiyayini Ramaswamy, Surya Suresh

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引用次数: 0

Abstract

Objective: This study investigates the biological activities of Rhododendron arboreum Sm from the eastern Himalayas, addressing a literature gap on its properties. It explores the plant's phytochemical, antioxidant, and medicinal characteristics.

Significance: Evaluating methanolic extracts of R. arboreum offers valuable insights into its bioactive potential. Comprehensive GC-MS analysis identified a diverse array of compounds, highlighting the plant's chemical composition.

Methods: Methanolic leaf and flower extracts underwent sequential extraction and phytochemical profiling using column chromatography, TLC, and GC-MS analysis. Spectral studies aided compound identification, and antioxidant activity was assessed via spectrophotometric assays.

Results: Column chromatography separated methanol leaf and flower extracts into 17 and 24 distinct fractions, respectively. TLC analysis showed specific R_f values for leaf (0.58, 0.65, 0.75, 0.8, 0.86, 0.9) and flower samples (0.91, 0.38, 0.48, 0.51, 0.56, 0.6, 0.65, 0.75, 0.85, 0.96). GC-MS analysis revealed a variety of organic functional groups, including aliphatic hydrocarbons, aromatic compounds, heterocyclic molecules, phenolic compounds, steroids, terpenoids, alcohols, esters, and other bioactive compounds. FTIR spectra identified functional groups such as hydroxyls, primary amines, alkanes, and alkynes. NMR data indicated a complex molecular composition with diverse proton environments. Leaf extracts demonstrated superior antioxidant activity compared to flower extracts in DPPH, ABTS, hydrogen peroxide scavenging, lipid peroxidation inhibition, and FRAP assays.

Conclusion: The study identifies diverse phytochemicals in R.arboreum extracts and highlights their potential applications in pharmaceuticals, nutraceuticals, and functional foods, owing to the superior antioxidant activity of leaf extracts compared to flowers.

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杜鹃花叶和花的植物化学成分分析和抗氧化评估：利用先进分析技术进行综合分析。

目的本研究调查了喜马拉雅山东部杜鹃花的生物活性，填补了有关其特性的文献空白。研究探讨了该植物的植物化学、抗氧化和药用特性。意义重大：评估 R. arboreum 的甲醇提取物为了解其生物活性潜力提供了宝贵的见解。全面的气相色谱-质谱（GC-MS）分析确定了一系列不同的化合物，突显了该植物的化学成分。研究方法对甲醇叶和花提取物进行顺序提取，并使用柱层析、TLC 和 GC-MS 分析进行植物化学成分分析。光谱研究有助于化合物鉴定，并通过分光光度法评估抗氧化活性。结果柱层析法将甲醇叶和花提取物分别分离成 17 和 24 个不同的馏分。TLC 分析显示叶片（0.58、0.65、0.75、0.8、0.86、0.9）和花朵样品（0.91、0.38、0.48、0.51、0.56、0.6、0.65、0.75、0.85、0.96）具有特定的 Rf 值。气相色谱-质谱分析显示了多种有机官能团，包括脂肪族碳氢化合物、芳香族化合物、杂环分子、酚类化合物、类固醇、萜类化合物、醇类、酯类和其他生物活性化合物。傅立叶变换红外光谱确定了羟基、伯胺、烷和炔等官能团。核磁共振数据表明其分子组成复杂，质子环境多样。在 DPPH、ABTS、过氧化氢清除、脂质过氧化抑制和 FRAP 试验中，叶提取物的抗氧化活性优于花提取物。结论由于叶提取物的抗氧化活性优于花提取物，该研究发现了 R.arboreum 提取物中的多种植物化学物质，并强调了它们在制药、营养保健品和功能食品中的潜在应用。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464