The effects of Cl− channel inhibitors and pyrethroid insecticides on calcium-activated chloride channels in neurons of Helicoverpa armigera

IF 3.9 3区 环境科学与生态学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Man Chen , Zixuan Ma , Jiayin Hou , Lingna Zhang , Hongmei Li-Byarlay , Bingjun He
{"title":"The effects of Cl− channel inhibitors and pyrethroid insecticides on calcium-activated chloride channels in neurons of Helicoverpa armigera","authors":"Man Chen ,&nbsp;Zixuan Ma ,&nbsp;Jiayin Hou ,&nbsp;Lingna Zhang ,&nbsp;Hongmei Li-Byarlay ,&nbsp;Bingjun He","doi":"10.1016/j.cbpc.2024.109999","DOIUrl":null,"url":null,"abstract":"<div><p>TMEM16A, a member of the Transmembrane protein 16 family, serves as the molecular basis for calcium activated chloride channels (CaCCs). We use RT-PCR to demonstrate the expression of TMEM16A in the neurons of <em>Helicoverpa armigera</em>, and record the CaCCs current of acute isolated neurons of <em>H. armigera</em> for the first time using patch clamp technology. In order to screen effective inhibitors of calcium-activated chloride channels, the inhibitory effects of four chloride channel inhibitors, CaCCinh-A01, NPPB, DIDS, and SITS, on CaCCs were compared. The inhibitory effects of the four inhibitors on the outward current of CaCCs were CaCCinh-A01 (10 μM, 56.31 %), NPPB (200 μM, 43.69 %), SITS (1 mM, 12.41 %) and DIDS (1 mM, 13.29 %). Among these inhibitors, CaCCinh-A01 demonstrated the highest efficacy as a blocker. To further explore whether calcium channel proteins can serve as potential targets of pyrethroids, we compared the effects of (type I) tefluthrin and (type II) deltamethrin on CaCCs. 10 μM and 100 μM tefluthrin can stimulate a large tail current in CaCCs, prolonging their deactivation time by 10.44 ms and 31.49 ms, and the V<sub>0.5</sub> shifted in the hyperpolarization by 2–8 mV. Then, deltamethrin had no obvious effect on the deactivation and activation of CaCCs. Therefore, CaCCs of <em>H. armigera</em> can be used as a potential target of pyrethroids, but type I and type II pyrethroids have different effects on CaCCs.</p></div>","PeriodicalId":10602,"journal":{"name":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","volume":"285 ","pages":"Article 109999"},"PeriodicalIF":3.9000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology C-toxicology & Pharmacology","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1532045624001674","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

TMEM16A, a member of the Transmembrane protein 16 family, serves as the molecular basis for calcium activated chloride channels (CaCCs). We use RT-PCR to demonstrate the expression of TMEM16A in the neurons of Helicoverpa armigera, and record the CaCCs current of acute isolated neurons of H. armigera for the first time using patch clamp technology. In order to screen effective inhibitors of calcium-activated chloride channels, the inhibitory effects of four chloride channel inhibitors, CaCCinh-A01, NPPB, DIDS, and SITS, on CaCCs were compared. The inhibitory effects of the four inhibitors on the outward current of CaCCs were CaCCinh-A01 (10 μM, 56.31 %), NPPB (200 μM, 43.69 %), SITS (1 mM, 12.41 %) and DIDS (1 mM, 13.29 %). Among these inhibitors, CaCCinh-A01 demonstrated the highest efficacy as a blocker. To further explore whether calcium channel proteins can serve as potential targets of pyrethroids, we compared the effects of (type I) tefluthrin and (type II) deltamethrin on CaCCs. 10 μM and 100 μM tefluthrin can stimulate a large tail current in CaCCs, prolonging their deactivation time by 10.44 ms and 31.49 ms, and the V0.5 shifted in the hyperpolarization by 2–8 mV. Then, deltamethrin had no obvious effect on the deactivation and activation of CaCCs. Therefore, CaCCs of H. armigera can be used as a potential target of pyrethroids, but type I and type II pyrethroids have different effects on CaCCs.

Abstract Image

Cl- 通道抑制剂和拟除虫菊酯杀虫剂对 Helicoverpa armigera 神经元中钙激活氯通道的影响。
TMEM16A是跨膜蛋白16家族的成员,是钙激活氯通道(CaCCs)的分子基础。我们利用 RT-PCR 技术证明了 TMEM16A 在蓟马神经元中的表达,并首次利用膜片钳技术记录了蓟马急性分离神经元的钙激活氯通道电流。为了筛选有效的钙激活氯离子通道抑制剂,比较了 CaCCinh-A01、NPPB、DIDS 和 SITS 四种氯离子通道抑制剂对 CaCCs 的抑制作用。四种抑制剂对 CaCCs 外向电流的抑制作用分别为 CaCCinh-A01 (10 μM,56.31%)、NPPB (200 μM,43.69%)、SITS (1 mM,12.41%)和 DIDS (1 mM,13.29%)。在这些抑制剂中,CaCCinh-A01 的阻断效力最高。为了进一步探讨钙通道蛋白是否可以作为拟除虫菊酯的潜在靶标,我们比较了(I 型)四氟菊酯和(II 型)溴氰菊酯对 CaCC 的影响。10 μM和100 μM的氟氯氰菊酯能刺激CaCCs产生较大的尾电流,使其失活时间分别延长10.44 ms和31.49 ms,超极化时V0.5偏移2-8 mV。而溴氰菊酯对CaCCs的失活和激活无明显影响。因此,拟除虫菊酯可作为除虫菊酯的潜在靶标,但Ⅰ型和Ⅱ型除虫菊酯对CaCCs的影响不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
7.50
自引率
5.10%
发文量
206
审稿时长
30 days
期刊介绍: Part C: Toxicology and Pharmacology. This journal is concerned with chemical and drug action at different levels of organization, biotransformation of xenobiotics, mechanisms of toxicity, including reactive oxygen species and carcinogenesis, endocrine disruptors, natural products chemistry, and signal transduction with a molecular approach to these fields.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信