Genotoxicity and Micronucleus Formation as a Result of Panoramic Radiography in Epithelial Cells of the Buccal Mucosa: A Cross-sectional Study in Adults

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2024-08-07 DOI:10.1002/cre2.915

Nakisa Torabinia, Mojdeh Mehdizadeh, Forooz Keshani, Mohammadreza Mehdizadeh, Parisa Soltani, Gianrico Spagnuolo

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引用次数: 0

Abstract

Objectives

To determine the genetic effects of panoramic radiography on the epithelial cells of the buccal mucosa by examining the micronucleus formation in these cells.

Materials and Methods

In this cross-sectional study, exfoliative cytology samples were prepared from the buccal mucosa of 36 patients immediately before and 10 days after panoramic radiography. The samples were prepared using liquid-based cytology with Papanicolaou staining. The slides were simultaneously evaluated by two expert pathologists and the ratio of the number of cells with micronuclei to the total number of cells on the slide was reported as a percentage. Data analysis was done using paired-samples T test, Pearson's correlation coefficient, and covariance analysis (α = 0.05).

Results

The study sample consisted of 24 (66.67%) males and 12 females (33.33%) with a mean (SD) age of 27.36 (8.19) years. The frequency of cells with micronucleus before and after panoramic radiography was not statistically different (p = 0.468). Additionally, the frequency of micronucleated cells was not correlated with age (p = 0.737) and sex (p = 0.211).

Conclusion

Panoramic exposure slightly increased the frequency of cells with micronucleus in epithelial cells of the buccal mucosa. However, this increase was not statistically significant.

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口腔粘膜上皮细胞全景放射摄影的遗传毒性和微核形成：一项成人横断面研究。

目的通过检测颊粘膜上皮细胞中微核的形成，确定全景放射摄影对这些细胞的遗传影响：在这项横断面研究中，从 36 名患者的颊粘膜上制备了脱落细胞学样本，分别在全景放射检查前和检查后 10 天进行。样本采用巴氏染色的液基细胞学方法制备。由两名病理专家同时对玻片进行评估，并以百分比的形式报告玻片上微核细胞数与细胞总数的比率。数据分析采用配对样本 T 检验、皮尔逊相关系数和协方差分析（α = 0.05）：研究样本中有 24 名男性（66.67%）和 12 名女性（33.33%），平均（标清）年龄为 27.36（8.19）岁。全景放射摄影前后微核细胞的频率没有统计学差异（P = 0.468）。此外，微核细胞的频率与年龄（p = 0.737）和性别（p = 0.211）无关：结论：全景暴露略微增加了口腔黏膜上皮细胞中微核细胞的频率。结论：全景曝光会略微增加口腔黏膜上皮细胞中的微核频率，但这种增加在统计学上并不显著。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.