Characterization of Inflammation/Immune-, Acute Phase-, Extracellular Matrix-, Adhesion-, and Serine Protease-Related Proteins in the Amniotic Fluid of Women With Early Preterm Prelabor Rupture of Membranes

IF 2.5 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology Pub Date : 2024-08-08 DOI:10.1111/aji.13913

Hyeon Ji Kim, Kyong-No Lee, Kyo Hoon Park, Bo Young Choi, Iseop Cho, Min Jung Lee

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Abstract

Problem

To determine whether altered concentrations of various inflammation/immune-, acute phase-, extracellular matrix-, adhesion-, and serine protease-related proteins in the amniotic fluid (AF) are independently associated with microbial invasion of the amniotic cavity and/or intra-amniotic inflammation (MIAC/IAI), imminent spontaneous preterm delivery (SPTD; ≤7 days), and major neonatal morbidity/mortality (NMM) in women with early preterm prelabor rupture of membranes (PPROM).

Method of Study

This was a retrospective cohort study involving 111 singleton pregnant women with PPROM (24–31 weeks) undergoing amniocentesis to diagnose MIAC/IAI. The following proteins were measured in stored AF samples by enzyme-linked immunosorbent assay (ELISA): APRIL, DKK-3, Gal-3BP, IGFBP-2, IL-8, VDBP, lumican, MMP-2, MMP-8, SPARC, TGFBI, TGF-β1, E-selectin, ICAM-5, P-selectin, haptoglobin, hepcidin, SAA1, kallistatin, and uPA.

Results

Multivariate logistic regression analyses revealed that (i) elevated APRIL, IL-8, MMP-8, and TGFBI levels in the AF, reduced lumican and SPARC levels in the AF, and high percentages of samples above the lower limit of quantification for AF TGF-β1 and uPA were significantly associated with MIAC/IAI; (ii) elevated AF levels of IL-8 and MMP-8 were significantly associated with SPTD within 7 days; and (iii) elevated AF IL-6 levels were significantly associated with increased risk for major NMM, when adjusted for baseline covariates.

Conclusion

ECM (lumican, SPRAC, TGFBI, and TGF-β1)- and serine protease (uPA)-associated proteins in the AF are involved in the regulation of the host response to infection/inflammation in the amniotic cavity, whereas AF inflammation (IL-8, MMP-8, and IL-6)-associated mediators are implicated in the development of preterm parturition and major NMM in early PPROM.

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早期早产胎膜早破妇女羊水中炎症/免疫、急性期、细胞外基质、粘附和丝氨酸蛋白酶相关蛋白的特征。

问题目的：确定羊水（AF）中各种炎症/免疫、急性期、细胞外基质、粘附和丝氨酸蛋白酶相关蛋白浓度的改变是否与微生物入侵羊膜腔和/或羊膜腔内炎症（MIAC/IAI）、即将发生的自发性早产（SPTD；≤7天）和早期早产胎膜早破（PPROM）产妇的主要新生儿发病率/死亡率（NMM）有关。研究方法：这是一项回顾性队列研究，涉及 111 名接受羊膜穿刺诊断 MIAC/IAI 的早产胎膜早破（24-31 周）单胎孕妇。通过酶联免疫吸附试验（ELISA）检测了储存的羊水样本中的以下蛋白质：APRIL、DKK-3、Gal-3BP、IGFBP-2、IL-8、VDBP、lumican、MMP-2、MMP-8、SPARC、TGFBI、TGF-β1、E-选择素、ICAM-5、P-选择素、血红蛋白、肝素、SAA1、kallistatin和uPA：多变量逻辑回归分析表明：(i)心房颤动中 APRIL、IL-8、MMP-8 和 TGFBI 水平升高，心房颤动中 lumican 和 SPARC 水平降低，以及超过心房颤动 TGF-β1 和 uPA 定量下限的样本百分比高与 MIAC/IAI 显著相关；(iii) 经基线协变量调整后，心房颤动 IL-6 水平升高与主要 NMM 风险增加显著相关。结论羊膜腔积液中的ECM（lumican、SPRAC、TGFBI和TGF-β1）和丝氨酸蛋白酶（uPA）相关蛋白参与调节宿主对羊膜腔感染/炎症的反应，而羊膜腔积液炎症（IL-8、MMP-8和IL-6）相关介质与早产和PPROM早期主要NMM的发生有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Reproductive Immunology 医学-免疫学

CiteScore

6.20

自引率

5.60%

发文量

314

审稿时长

2 months

期刊介绍： The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.