MXene-Polydopamine-antiCEACAM1 Antibody Complex as a Strategy for Targeted Ablation of Melanoma.

IF 8.2 2区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
ACS Applied Materials & Interfaces Pub Date : 2024-08-21 Epub Date: 2024-08-07 DOI:10.1021/acsami.4c08129
Anastasia Konieva, Volodymyr Deineka, Kateryna Diedkova, Daniel Aguilar-Ferrer, Mykola Lyndin, Gunther Wennemuth, Viktoriia Korniienko, Sergiy Kyrylenko, Alexey Lihachev, Veronika Zahorodna, Ivan Baginskiy, Emerson Coy, Oleksiy Gogotsi, Agata Blacha-Grzechnik, Wojciech Simka, Irina Kube-Golovin, Igor Iatsunskyi, Maksym Pogorielov
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引用次数: 0

Abstract

Photothermal therapy (PTT) is a method for eradicating tumor tissues through the use of photothermal materials and photosensitizing agents that absorb light energy from laser sources and convert it into heat, which selectively targets and destroys cancer cells while sparing healthy tissue. MXenes have been intensively investigated as photosensitizing agents for PTT. However, achieving the selectivity of MXenes to the tumor cells remains a challenge. Specific antibodies (Ab) against tumor antigens can achieve homing of the photosensitizing agents toward tumor cells, but their immobilization on MXene received little attention. Here, we offer a strategy for the selective ablation of melanoma cells using MXene-polydopamine-antiCEACAM1 Ab complexes. We coated Ti3C2Tx MXene with polydopamine (PDA), a natural compound that attaches Ab to the MXene surface, followed by conjugation with an anti-CEACAM1 Ab. Our experiments confirm the biocompatibility of the Ti3C2Tx-PDA and Ti3C2Tx-PDA-antiCEACAM1 Ab complexes across various cell types. We also established a protocol for the selective ablation of CEACAM1-positive melanoma cells using near-infrared irradiation. The obtained complexes exhibit high selectivity and efficiency in targeting and eliminating CEACAM1-positive melanoma cells while sparing CEACAM1-negative cells. These results demonstrate the potential of MXene-PDA-Ab complexes for cancer therapy. They underline the critical role of targeted therapies in oncology, offering a promising avenue for the precise and safe treatment of melanoma and possibly other cancers characterized by specific biomarkers. Future research will aim to refine these complexes for clinical use, paving the way for new strategies for cancer treatment.

Abstract Image

MXene-Polydopamine-antiCEACAM1 抗体复合物作为靶向消融黑色素瘤的一种策略。
光热疗法(PTT)是一种通过使用光热材料和光敏剂来消除肿瘤组织的方法,光热材料和光敏剂可吸收激光源的光能并将其转化为热能,从而选择性地靶向摧毁癌细胞,同时保护健康组织。人们一直在深入研究将 MXenes 用作 PTT 的光敏剂。然而,实现 MXenes 对肿瘤细胞的选择性仍然是一项挑战。针对肿瘤抗原的特异性抗体(Ab)可以实现光敏剂对肿瘤细胞的归巢,但将其固定在 MXene 上却很少受到关注。在此,我们提出了一种利用 MXene-多巴胺-抗 CEACAM1 抗体复合物选择性消融黑色素瘤细胞的策略。我们在 Ti3C2Tx MXene 表面涂上了聚多巴胺(PDA),这是一种天然化合物,可将抗体附着在 MXene 表面,然后再与抗 CEACAM1 抗体共轭。我们的实验证实了 Ti3C2Tx-PDA 和 Ti3C2Tx-PDA-antiCEACAM1 Ab 复合物在各种细胞类型中的生物相容性。我们还建立了一种利用近红外照射选择性消融 CEACAM1 阳性黑色素瘤细胞的方案。所获得的复合物在靶向和消除 CEACAM1 阳性黑色素瘤细胞方面表现出高度的选择性和高效性,而 CEACAM1 阴性细胞则不受影响。这些结果证明了 MXene-PDA-Ab 复合物在癌症治疗方面的潜力。它们强调了靶向疗法在肿瘤学中的关键作用,为精确、安全地治疗黑色素瘤以及其他可能具有特定生物标志物特征的癌症提供了一条前景广阔的途径。未来的研究将致力于完善这些复合物的临床应用,为癌症治疗的新策略铺平道路。
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来源期刊
ACS Applied Materials & Interfaces
ACS Applied Materials & Interfaces 工程技术-材料科学:综合
CiteScore
16.00
自引率
6.30%
发文量
4978
审稿时长
1.8 months
期刊介绍: ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.
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