Chondrogenic potential of superficial versus cartilage layer cells of the temporomandibular joint condyle in photopolymerizable gelatin-based hydrogels.

IF 1.7 4区 医学 Q3 ENGINEERING, BIOMEDICAL
Wuyang Li, Juan M Taboas, Alejandro J Almarza
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引用次数: 0

Abstract

The objectives of this study were to compare the chondrogenic potential of cells derived from different layers of Mandibular condyle cartilage and to gain further understanding of the impact of chondrogenic cues when embedded into a novel hydrogel scaffold (PGH, a polymer blend of poly (ethylene glycol), gelatin, and heparin) compared to a gelatin hydrogel scaffold (GEL). Cartilage layer cells (CLCs) and fibroblastic superficial layer cells (SLCs) were harvested from the mandibular condyle of boer goats obtained from a local abattoir. After expansion, cells were seeded into PGH and GEL hydrogels and cultured in chondrogenic media for 3 weeks. Scaffolds were harvested at 0, 1, and 3 week(s) and processed for gross appearance, histochemical, biochemical, and mechanical assays. In terms of chondrogenesis, major differences were observed between scaffold materials, but not cell types. Glycosaminoglycan (GAG) staining showed GEL scaffolds deposited GAG during the 3 week period, which was also confirmed with the biochemical testing. Moreover, GEL scaffolds had significantly higher compressive modulus and peak stress than PGH scaffolds at all time points with the largest difference seen in week 3. It can be concluded that GEL outperformed PGH in chondrogenesis. It can also be concluded that materials play a more important role in the process of chondrogenesis than the tested cell populations. Fibroblastic SLCs were shown to have similar chondrogenic potential as CLCs cells, suggesting a rich pool of progenitor cells in the superficial fibroblastic layer capable of undergoing chondrogenesis given appropriate physical and chemical cues.

颞下颌关节髁状突表层细胞与软骨层细胞在光聚合明胶水凝胶中的软骨生成潜能。
本研究的目的是比较来自下颌骨髁状突软骨不同层的细胞的软骨生成潜能,并进一步了解与明胶水凝胶支架(GEL)相比,嵌入新型水凝胶支架(PGH,聚乙二醇、明胶和肝素的聚合物混合物)的软骨生成线索的影响。软骨层细胞(CLC)和成纤维浅层细胞(SLC)取自当地屠宰场的野山羊下颌髁状突。扩增后,将细胞播种到 PGH 和 GEL 水凝胶中,并在软骨培养基中培养 3 周。分别在第0周、第1周和第3周时收获支架,并对其进行外观、组织化学、生物化学和机械测定。在软骨形成方面,不同支架材料之间存在重大差异,但细胞类型之间没有差异。糖胺聚糖(GAG)染色显示 GEL 支架在 3 周内沉积了 GAG,生化测试也证实了这一点。此外,在所有时间点上,GEL 支架的压缩模量和峰值应力都明显高于 PGH 支架,其中第 3 周的差异最大。由此可以得出结论,GEL 在软骨生成方面的表现优于 PGH。此外,还可以得出结论,在软骨形成过程中,材料比测试的细胞群发挥着更重要的作用。成纤维细胞 SLCs 与 CLCs 细胞具有相似的软骨生成潜能,这表明表层成纤维细胞中有丰富的祖细胞,在适当的物理和化学因素作用下,它们能够进行软骨生成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
3.60
自引率
5.60%
发文量
122
审稿时长
6 months
期刊介绍: The Journal of Engineering in Medicine is an interdisciplinary journal encompassing all aspects of engineering in medicine. The Journal is a vital tool for maintaining an understanding of the newest techniques and research in medical engineering.
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