Adaptability of the gut microbiota of the German cockroach Blattella germanica to a periodic antibiotic treatment

IF 6.1 1区 生物学 Q1 MICROBIOLOGY
Jesús Marín-Miret , Ana Elena Pérez-Cobas , Rebeca Domínguez-Santos , Benjamí Pérez-Rocher , Amparo Latorre , Andrés Moya
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Abstract

High-throughput sequencing studies have shown that diet or antimicrobial treatments impact animal gut microbiota equilibrium. However, properties related to the gut microbial ecosystem stability, such as resilience, resistance, or functional redundancy, must be better understood. To shed light on these ecological processes, we combined advanced statistical methods with 16 S rRNA gene sequencing, functional prediction, and fitness analyses in the gut microbiota of the cockroach Blattella germanica subject to three periodic pulses of the antibiotic (AB) kanamycin (n=512). We first confirmed that AB did not significantly affect cockroaches' biological fitness, and gut microbiota changes were not caused by insect physiology alterations. The sex variable was examined for the first time in this species, and no statistical differences in the gut microbiota diversity or composition were found. The comparison of the gut microbiota dynamics in control and treated populations revealed that (1) AB treatment decreases diversity and completely disrupts the co-occurrence networks between bacteria, significantly altering the gut community structure. (2) Although AB also affected the genetic composition, functional redundancy would explain a smaller effect on the functional potential than on the taxonomic composition. (3) As predicted by Taylor's law, AB generally affected the most abundant taxa to a lesser extent than the less abundant taxa. (4) Taxa follow different trends in response to ABs, highlighting "resistant taxa," which could be critical for community restoration. (5) The gut microbiota recovered faster after the three AB pulses, suggesting that gut microbiota adapts to repeated treatments.

德国蜚蠊肠道微生物群对定期抗生素治疗的适应性。
高通量测序研究表明,饮食或抗菌治疗会影响动物肠道微生物群的平衡。然而,必须更好地了解与肠道微生物生态系统稳定性相关的特性,如恢复力、抵抗力或功能冗余。为了揭示这些生态过程,我们将先进的统计方法与 16 S rRNA 基因测序、功能预测和适应性分析相结合,研究了受到三种周期性抗生素(AB)卡那霉素影响的德国蜚蠊(n=512)的肠道微生物群。我们首先确认,抗生素对蟑螂的生物适应性没有明显影响,肠道微生物群的变化不是由昆虫生理变化引起的。我们首次对该物种的性别变量进行了研究,结果发现肠道微生物群的多样性和组成没有统计学差异。对照种群和处理种群的肠道微生物群动态比较显示:(1)AB 处理降低了多样性,完全破坏了细菌之间的共生网络,显著改变了肠道群落结构。(2)虽然 AB 也影响遗传组成,但功能冗余对功能潜力的影响小于对分类组成的影响。(3) 正如泰勒定律所预测的那样,AB 对数量最多的类群的影响一般小于数量较少的类群。(4) 分类群对 AB 的反应趋势不同,突出了 "抗性分类群",这可能对群落恢复至关重要。(5)肠道微生物群在三次AB脉冲后恢复较快,表明肠道微生物群能适应重复处理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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