Development of a peptide-generated antibody to rabbit hemorrhagic disease virus 2 VP60 and its immunohistochemical application in natural cases.

IF 1.2 3区农林科学 Q3 VETERINARY SCIENCES

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-09-01 Epub Date: 2024-08-06 DOI:10.1177/10406387241265370

Alisia A W Weyna, Natalie K Stilwell, Rachel Anders, Brittany McHale, Rita McManamon, Elizabeth W Howerth

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引用次数: 0

Abstract

Rabbit hemorrhagic disease virus 2 (RHDV2) has spread across the United States infecting and causing death in domestic and wild rabbits. Immunohistochemistry (IHC) would be a useful tool for the detection of RHDV2 antigen in tissues as it is inexpensive and readily achievable in most diagnostic laboratories. However, there is no readily available antibody for this purpose. To fill this void, we generated an RHDV2 capsid protein VP60-specific antibody in chicken eggs and validated the antibody using formalin-fixed tissues from 5 domestic rabbits naturally infected with RHDV2. Viral antigen was detected immunohistochemically in various tissues, most prominently in hepatocytes and macrophages in liver, and in macrophages in spleen and cecal lymphoid tissue. Intravascular mononuclear cells in lung and renal tubular and biliary epithelium also were immunolabeled. Both nuclear and cytoplasmic immunolabeling were observed. This peptide-generated antibody is a potentially useful tool as an adjunct to reverse-transcription PCR or in situ hybridization for detection of RHDV2 in tissues.

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兔出血性疾病病毒 2 VP60 多肽抗体的开发及其在自然病例中的免疫组化应用。

兔出血性疾病病毒 2（RHDV2）已在美国蔓延，感染家兔和野兔并导致其死亡。免疫组织化学（IHC）是检测组织中 RHDV2 抗原的有用工具，因为它价格低廉，而且在大多数诊断实验室中都很容易实现。然而，目前还没有可用于此目的的抗体。为了填补这一空白，我们在鸡卵中生成了一种RHDV2囊膜蛋白VP60特异性抗体，并用5只自然感染RHDV2的家兔的福尔马林固定组织对该抗体进行了验证。免疫组织化学方法在不同组织中检测到了病毒抗原，其中最显著的是肝脏中的肝细胞和巨噬细胞，以及脾脏和盲肠淋巴组织中的巨噬细胞。肺、肾小管和胆道上皮细胞中的血管内单核细胞也被免疫标记。可观察到细胞核和细胞质免疫标记。这种多肽生成的抗体可作为反转录 PCR 或原位杂交的辅助工具，用于检测组织中的 RHDV2。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Diagnostic Investigation 农林科学-兽医学

CiteScore

3.00

自引率

6.70%

发文量

127

审稿时长

6-16 weeks

期刊介绍： The Journal of Veterinary Diagnostic Investigation (J Vet Diagn Invest) is an international peer-reviewed journal published bimonthly in English by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). JVDI is devoted to all aspects of veterinary laboratory diagnostic science including the major disciplines of anatomic pathology, bacteriology/mycology, clinical pathology, epidemiology, immunology, laboratory information management, molecular biology, parasitology, public health, toxicology, and virology.